SVCT

94122105, human breast, Epithelial

• Sinónimos: SCTV, SCVT
• UNSPSC Code: 41106514

Propiedades

• product name: SVCT, 94122105, from human breast
• biological source: human breast
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice

Descripción

Cell Line Origin

Human breast epithelium, SV40 transformed

Cell Line Description

SVCT has been established from human breast epithelial cells being transfected with SV40. The cell line shows an abnormal karyotype and was not tumourigenic in athymic male nude mice. SVCT have been found positive for the expression of keratin 7, 8 and 18 but negative for keratin 19. Epithelial cells, dome formation when confluent.

DNA Profile

Not specified

Culture Medium

DMEM + 2 mM Glutamine + 5μg/ml Hydrocortisone + 10μg/ml Insulin + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4, i.e., seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Other Notes

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Disclaimer

This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.