2fTGH cell line human

12021508, human fibrosarcoma, Epithelial

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: 2fTGH cell line human, 12021508
• origen biológico: human fibrosarcoma
• modo de crecimiento: adherent
• morfología: Epithelial
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Descripción línea celular

The human sarcoma cell line HT 1080 (ECACC Catalogue number 85111505) was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by alpha interferon to create the 2fTGH cell line, enabling the selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the parent 2fTGH cell line enabled the isolation of a number of IFNg signalling mutants available from ECACC: U2A (12021502), U3A (12021503), U4A (12021504), U4C (12021505), U5A (12021506), U6A (12021507). Each contains a different mutation in the IFN signalling pathway. The 2fTGH panel provides a tool for the in vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway.

Perfil del ADN

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19

Medio de cultivo

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4 x 10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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Cláusula de descargo de responsabilidad

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.