UM-UC-13

8090508, human bladder, Epithelial

• Sinónimos: Human UM-UC-13, UM-UC-13 Cell Line
• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: UM-UC-13, 08090508
• origen biológico: human bladder
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: metastasis
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human transitional cell carcinoma lymphatic metastasis

Descripción línea celular

UM-UC-13 was produced from a lymphatic metastasis from a transitional cell carcinoma of the bladder. UM-UC-13 produced tumours in female athymic nude mice after 36 days. Susceptibility to adenoviral mediated gene transduction and expression of p53, p16, retinoblastoma protein and coxsackie adenovirus receptor were studied.

Perfil del ADN

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8,12
D16S539: 11
D5S818: 9,13
D7S820: 8,9
THO1: 9
TPOX: 8
vWA: 16,18

Medio de cultivo

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37 °C.

Otras notas

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Cláusula de descargo de responsabilidad

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.