C7438

Chaperonin 10 from Escherichia coli

≥95.0% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder

• Sinónimos: GroES
• Número MDL: MFCD00163256
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• origen biológico: Escherichia coli
• Nivel de calidad: 200
• recombinante: expressed in E. coli overproducing strain
• Ensayo: ≥95.0% (SDS-PAGE)
• Formulario: lyophilized powder
• técnicas: activity assay: suitable
• Nº de acceso UniProt: P61604
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: human ... HSPE1(3336)

Descripción

Acciones bioquímicas o fisiológicas

Chaperonin60 (GroEL) and chaperonin10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles. The chaperonins assist the folding of nascent, organelle-imported or stress-destabilized polypeptides. In vitro, purified GroEL together with purified GroES in the presence of Mg-ATP facilitate refolding and reactivation of denatured proteins, e.g., the photosynthetic enzyme rubisco and the mitochondrial enzyme rhodanese.
The folding activity of a 1:1 molar mixture of GroEL and GroES was tested using urea-denatured rhodanese. At least 2-fold reactivation of rhodanese over the spontaneous reactivation was obtained.

Envase

Package size based on protein content.

Forma física

Lyophilized powder containing Tris buffer salts, potassium chloride, dithiothreitol and trehalose as stabilizer.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)