C3483
Cytochrome c from human heart
≥95% (SDS-PAGE), lyophilized powder
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origen biológico
human heart
Nivel de calidad
Ensayo
≥95% (SDS-PAGE)
Formulario
lyophilized powder
mol peso
11 kDa by SDS-PAGE
técnicas
cell culture | mammalian: suitable
mass spectrometry (MS): suitable
western blot (chemiluminescent): suitable
Nº de acceso UniProt
temp. de almacenamiento
−20°C
Información sobre el gen
human ... CYCS(54205) , CYCSL1(157317)
Descripción general
Cytochrome c (CYCS) is mapped to human chromosome 7p15.3.[1] CYCS is a heme protein containing three long α-helices. It undergoes domain swapping at the C-terminus and exists in dimeric, trimeric, and tetrameric forms.[2] Insertion mutations in CYCS is implicated in acute myeloid leukemia.[1] Mutations in CYCS modulates the release of proplatelets and decreases platelet production leading to thrombocytopenia 4.[3]
Aplicación
Cytochrome c from human heart has been used for the generation of standard curves in the electrochemiluminescence assay for measurement of cytochrome C levels in plasma samples[4] and in electrospray ionization mass spectrum (ESI-MS) analysis.[5]
Cytochrome c has been identified as an important mediator in apoptotic pathways. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell. Investigation on the effect of Paris Saponin I (PS I) on human gastric carcinoma cell growth (SGC7901 cells) have shown an elevated level cytoplasmic cytochrome c. Results are an inhibition of proliferation in SGC7901 cells by inducing mitochondria-dependent apoptosis through cytochrome c.
Acciones bioquímicas o fisiológicas
Cytochrome c is a small heme protein that is a mobile electron carrier in the mitochondrial electron transport chain. It is also an initiator of apoptosis.
The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen.
The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen.
In addition to its function in electron transport, cytochrome c acts as an apoptosis-triggering agent, forming the apoptosome with apoptosis protease-activating factor-1 (Apaf-1) and activating the caspase cascade. The two functions are finely tuned by phosphorylation of Tyr residues at position 48 and/or 97. One recent report provides suggestive evidence that phosphorylation at Tyr48 switches off the apoptotic effect.
In addition to its function in electron transport, cytochrome c acts as an apoptosis-triggering agent, forming the apoptosome with apoptosis protease-activating factor-1 (Apaf-1) and activating the caspase cascade. The two functions are finely tuned by phosphorylation of Tyr residues at position 48 and/or 97. One recent report provides suggestive evidence that phosphorylation at Tyr48 switches off the apoptotic effect.
Forma física
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.
Nota de preparación
Chemically oxidized during purification.
Otras notas
View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.
Aplicación
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
13 - Non Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Lin-Lin Gao et al.
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To investigate the anti-tumor effects of Paris chinensis dioscin (PCD) and mechanisms regarding cell cycle regulation and apoptosis in human gastric cancer SGC-7901 cells. Cell viability was analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. Cell apoptosis was evaluated by flow cytometry
Chromatograms
application for HPLCapplication for HPLCapplication for HPLCActive Filters
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