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B3111

ANTI-FLAG® M2 antibody, Mouse monoclonal

Clone M2, purified from hybridoma cell culture in bioreactor

Sinónimos:

Anti-ddddk, Anti-dykddddk, M2 clone ANTI-FLAG

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NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
M2, monoclonal
Technique(s):
FLAG purification: suitable
Application:
FLAG purification
Citations:
19
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biological source

mouse

antibody form

purified immunoglobulin (purified IgG1 subclass)

clone

M2, monoclonal

shelf life

4 yr

purified by

using Protein A

technique(s)

FLAG purification: suitable

storage temp.

−20°C

General description

Monoclonal ANTI-FLAG M2 is a purified immunoglobulin, IgG1, monoclonal antibody, purified from culture supernatant of hybridoma cells, that binds to FLAG® fusion proteins. Unlike ANTI-FLAG M1 antibody, the M2 antibody will recognize the FLAG sequence at the N-terminus, Met-N-terminus, C-terminus, or at an internal site of FLAG fusion proteins. Monoclonal ANTI-FLAG M2 is useful for identification and capture of FLAG fusion proteins by common immunological procedures such as Western blots and immunoprecipitation. It is also useful for affinity purification of FLAG fusion proteins when bound to a solid support.

form: solution pH 7.4, containing 15 mM sodium azide
concentration: 3.0-5.0 mg/mL

Application

IB, IF, IP, FACS, ELISA
Antibody is recommended for use in several applications such as immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, and ELISA.

Learn more product details in our FLAG® application portal.

Packaging

polypropylene screw cap vial

Preparation Note

Dilute the antibody solution from 0.5-10 ug/mL in specified buffer

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Este artículo
F3165F1804M8823
description

Clone M2, purified from hybridoma cell culture in bioreactor

description

clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

description

1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

description

affinity isolated antibody

antibody form

purified immunoglobulin (purified IgG1 subclass)

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shelf life

4 yr

shelf life

-

shelf life

-

shelf life

2 yr at -20 °C

purified by

using Protein A

purified by

using Protein A

purified by

affinity chromatography (affinity tag purification)

purified by

-


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