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Merck

27816

Sigma-Aldrich

Brilliant Blue R

250, for microscopy

Sinónimos:

Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R

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About This Item

Fórmula empírica (notación de Hill):
C45H44N3NaO7S2
Número de CAS:
Peso molecular:
825.97
Colour Index Number:
42660
Beilstein/REAXYS Number:
5718025
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.26

grade

for microscopy

Quality Level

form

powder

λmax

589.2 nm in ethanol

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

InChI key

NKLPQNGYXWVELD-UHFFFAOYSA-M

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Application

For detection of protein bands following electrophoresis.

Other Notes

Protein stain for gel electrophoresis; 5 to 10 times more sensitive than Naphthol Blue Black B and Bromophenol (as little as 0.5 μg/cm2 of protein could be detected in a gel matrix)

Legal Information

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Biochimica et biophysica acta, 71, 377-391 (1963-05-14)
Two new procedures are described for the estimation of protein by direct photometry on electrophoretic strips. The protein complexes of procion brilliant blue RS and coomassie brilliant blue R250 are shown to follow Beer's law up to 50 and 20

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