Saltar al contenido
Merck
Todas las fotos(1)

Documentos

11663925910

Roche

Mycoplasma PCR ELISA

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

UNSPSC Code:
12352200

usage

sufficient for 96 reactions

manufacturer/tradename

Roche

shipped in

dry ice

storage temp.

−20°C

Specificity

The Mycoplasma PCR ELISA is known to detect all of the following mycoplasma, acholeplasma, and ureaplasma species: M. orale, M. arginini, M. fermentans, M. hyorhinis, M. salivarium, M. gallisepticum, M. hominis, M. bovis, M. californicum, M. bovoculi, M. Pg50 bovine group, M. bovigenitalium, M. hyopneumoniae, A. laidlawii, U. urealyticum. DNA from other bacteria (except for Clostridium sp.), yeast, and eukaryotic cells are not detected.

Application

For research use only. Not for use in diagnostic procedures.
Mycoplasma PCR ELISA kit has been used for the detection of contamination by mycoplasma/acholeplasma/ureaplasma in cell cultures or other sample materials.
Mycoplasma PCR ELISA has been used to test mycoplasma contamination in cell lines.

Features and Benefits

Assay time: approximately 6 hours
Sample material: Cell culture supernatants, cell suspensions, or other samples
Sensitivity: At least 1 x 103 colony-forming units (cfu) of mycoplasma per milliliter of culture medium
  • High sensitivity: Detects 1 - 10fg of DNA, corresponding to approximately 1 - 20 gene copies per reaction (at least 103cfu/ml)
  • Broad detection range: All mycoplasma, acholeplasma, and ureaplasma species tested thus far were detected
  • Simplicity: Detects all mycoplasma using the same protocol, as opposed to the culture method that requires different culture conditions for different mycoplasma species
  • Fast results: The entire procedure can be performed in one day
  • Easy handling of large numbers of samples: The ready-to-use mix and ELISA detection procedure allow fast and semi-automatic sample processing

Packaging

1 kit containing 14 components.

Sequence

Chain Length Positive control: 520 bp.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Solo componentes del kit

Referencia del producto
Descripción
  • Lysis Reagent
  • Neutralization Reagent
  • PCR Mix ready-to-use
  • Denaturation Reagent
  • Hybridization Buffer
  • Biotinylated Capture Probe
  • Washing Buffer 10x concentrated
  • Anti-Digoxigenin-POD antibody
  • Conjugate Dilution Buffer
  • TMB Substrate Solution
  • Stop Reagent
  • Positive Control DNA
  • Streptavidin-coated Microplate
  • Adhesive Plate Cover Foils
Ver todo (14)

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Los clientes también vieron

Slide 1 of 2

1 of 2

Mycoplasma agar base suitable for microbiology, NutriSelect® Plus

Millipore

M2178

Mycoplasma agar base

bisBenzimide H 33258 powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC)

Sigma-Aldrich

B1155

bisBenzimide H 33258

Novel Non-phosphorylated Serine 9/21 GSK3?/? Antibodies: Expanding the Tools for Studying GSK3 Regulation
Grabinski T, et al.
Frontiers in Molecular Neuroscience, 9, 123-123 (2016)
Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi
Margaryan N V, et al.
International Journal of Molecular Sciences, 17(3), 418-418 (2016)
Kunal Karve et al.
Cell death & disease, 11(8), 704-704 (2020-08-28)
Alternative splicing contributes to diversification of gene function, yet consequences of splicing on functions of specific gene products is poorly understood. The major transcription factor TCF7L2 undergoes alternative splicing but the biological significance of TCF7L2 isoforms has remained largely to
Pengfei Wang et al.
Nature, 593(7857), 130-135 (2021-03-09)
The COVID-19 pandemic has had widespread effects across the globe, and its causative agent, SARS-CoV-2, continues to spread. Effective interventions need to be developed to end this pandemic. Single and combination therapies with monoclonal antibodies have received emergency use authorization1-3
Novel Non-phosphorylated Serine 9/21 GSK3?/a Antibodies: Expanding the Tools for Studying GSK3 Regulation
Tessa G, et al.
Frontiers in Molecular Neuroscience (2016)
Ver todos los artículos relacionados

Artículos

Mycoplasma PCR ELISA Protocol

Mycoplasmas are potential contaminants in mammalian cell culture manufacturing. All products produced in cell culture to be tested for the presence of Mycoplasma

Protocolos

Optimized PCR-based Detection of Mycoplasma

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico