10602400001

Thrombin

from human plasma

• Sinónimos: coagulation factor Iia, thrombin
• Comisión internacional de enzimas: 3.4.21.5 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propiedades

• origen biológico: human plasma
• Nivel de calidad: 100
• Formulario: lyophilized
• actividad específica: ~120 units/mg protein (At 25 °C with Chromozym TH as the substrate.)
• mol peso: M_r ~33.6 kDa
• envase: pkg of 20 U
• fabricante / nombre comercial: Roche
• pH óptimo: 8.2-9.0
• Condiciones de envío: wet ice

Descripción

Descripción general

Thrombin is a Na⁺ activated allosteric serine protease. It belongs to chymotrypsin family.[1] Thrombin is made of two polypeptide chains of 36 (A chain) and 259 (B chain) residues that are covalently attached with a disulfide bond.[1]

Thrombin is a coagulation factor IIa.[2] It is a serine endopeptidase that hydrolyzes peptide and ester bonds specifically at the carboxylic side of Arg.[3] The enzyme converts fibrinogen to fibrin.[4] The product contains EDTA and additional stabilizing agents.

Aplicación

Thrombin has been used in the expression and purification of recombinant RELM (resistin-like moleculefamily members.[5] It has also been used in platelet spreading on fibrinogen[6][7] and immunostaining.[8]

Thrombin is used in coagulation research, medical research, protein-structure analysis, and biochemical research. It has been used for the stimulation of the platelets.[8][9]

Acciones bioquímicas o fisiológicas

Thrombin plays an important role in blood coagulation. It serve as a procoagulant factor while transforming fibrinogen into an insoluble fibrin clot. Thrombin can act as anticoagulant by the activation of protein C.[1]

Forma física

Lyophilizate

Nota de preparación

Storage conditions (working solution): -15 to -25°C

Inhibitors: DFP, TLCK, PMSF, benzamidine, α₁-antitrypsin, α₂-macroglobulin, antithrombin III heparin, hirudin, and APMSF

Reconstitución

The reconstituted solution contains 20 mM EDTA.
Important note:
Use plastic vials and pipettes because thrombin will be adsorbed to glass surfaces!

Almacenamiento y estabilidad

Store at 2 to 8 °C. (Store dry!)

Otras notas

For life science research only. Not for use in diagnostic procedures.

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Información de seguridad

• Pictogramas: GHS07,GHS08
• Palabra de señalización: Warning
• Frases de peligro: H332,H373
• Consejos de prudencia: P260 - P271 - P304 + P340 + P312 - P314 - P501
• Clasificaciones de peligro: Acute Tox. 4 Inhalation - STOT RE 2
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash