10280879001
Roche
5-Bromo-2′-desoxiuridina
>97%, crystalline solid, pkg of 1 g
Sinónimos:
5- BrdU, 5-Bromo-1-(2-desoxi-β-D-ribofuranosil)uracilo, BUdR, Desoxirribósido de 5-bromouracilo
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About This Item
Fórmula empírica (notación de Hill):
C9H11BrN2O5
Número de CAS:
Peso molecular:
307.10
Beilstein:
30395
Número MDL:
Código UNSPSC:
12352204
ID de la sustancia en PubChem:
Productos recomendados
Nivel de calidad
Ensayo
>97%
Formulario
crystalline solid
mol peso
307.1
envase
pkg of 1 g
fabricante / nombre comercial
Roche
mp
191-194 °C (dec.) (lit.)
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
cadena SMILES
OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
InChI
1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
Clave InChI
WOVKYSAHUYNSMH-RRKCRQDMSA-N
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Descripción general
It is conventionally used as a marker for S-phase synthesis of DNA.
Aplicación
5-Bromo-2′-deoxyuridine has been used to label cells in the S-phase of the cell cycle and primary hepatocytes during cell proliferation studies.
Immunochemically detectable nucleotide that can be incorporated into DNA instead of thymidine. Used for in vivo studies of DNA synthesis and for the detection of mutagenic substances, according to the sister chromatid exchange (SCE) method.
Acciones bioquímicas o fisiológicas
Análogo de timidina utilizado como mutágeno en investigación genética. Incorporada de manera selectiva en el ADN celular durante la fase S.
Calidad
Purity: 97% (from N), chromatographically homogeneous
Nota de preparación
Working concentration: According to literature for cell culture, a final concentration of 10 μmol/l has been used.
According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.
Preparation of Working Solution
Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2′-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.
Storage conditions (working solution): -15 to -25 °C
According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.
Preparation of Working Solution
Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2′-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.
Storage conditions (working solution): -15 to -25 °C
Otras notas
For life science research only. Not for use in diagnostic procedures.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Muta. 1B - Repr. 2
Código de clase de almacenamiento
13 - Non Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Yukinobu Goto et al.
American journal of physiology. Cell physiology, 285(2), C253-C259 (2003-04-04)
The present study was designed to develop methods to study the production and release of monocytes from the bone marrow using the thymidine analog 5'-bromo-2'-deoxyuridine (BrdU). Dividing monocytes in bone marrow were labeled with BrdU (MOBrdU), and their release into
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The American Journal of Pathology, 189(2), 258-271 (2019)
K M Connolly et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 41(1), 1-6 (1993-01-01)
Proliferating cell nuclear antigen (PCNA) was evaluated as a marker of cell proliferation in formalin-fixed rat liver tissue through a comparative study with the thymidine analogue 5-bromo-2'-deoxyuridine (BrdU). The comparison was conducted through the introduction of a dual immunohistochemical procedure
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