GF05

Anti-Granulocyte Colony Stimulating Factor (Ab-1) Mouse mAb (5.24)

liquid, clone 5.24, Calbiochem^®

• Sinónimos: Anti-G-CSF
• Código UNSPSC: 12352203

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 5.24, monoclonal
• Formulario: liquid
• contiene: ≤0.1% sodium azide as preservative
• reactividad de especies: human
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: do not freeze
• isotipo: IgG2
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CSF3(1440)

Descripción

Descripción general

Anti-Granulocyte Colony Stimulating Factor (Ab-1) mouse monoclonal, clone 5.24, recognizes ~19 kDa G-CSF in bone marrow & fetal liver tissue. It is validated for use in WB, IF & IHC (paraffin).

Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes granulocyte colony stimulating factor.

Recognizes the ~19 kDa G-CSF glycoprotein in bone marrow and fetal liver tissue.

Inmunógeno

Human

purified human G-CSF

Aplicación

Immunoblotting (1-5 µg/ml)

Immunofluorescence (1-5 µg/ml)

Paraffin Sections (1-10 µg/ml, saponin pre-treatment required, see comments)

Envase

Please refer to vial label for lot-specific concentration.

Advertencia

Toxicity: Standard Handling (A)

Forma física

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Nota de análisis

Positive Control
Recombinant G-CSF protein, bone marrow tissue, or fetal liver tissue

Otras notas

For paraffin sections, saponin treatment is required for antigen retrieval. Antibody should be titrated for optimal results in individual systems.

Nicola, N.A. 1987. J. Cell Cloning5, 1.
Metcalf, D., 1986. Blood67, 257.
Nagata, S., et al. 1986. Nature319, 415.
Nagata, S., et al. 1986 EMBO J.5, 575.
Souza, L.M., et al. 1986 Science232, 61.
Metcalf, D. 1984, Hemopoietic colony stimulating factors. Amsterdam: Elsevier.
Nicola, N.A. Vadas, M.A., 1984. Immunol Today5, 76.
Nicola, N.A., et al. 1983 J. Biol. Chem.258, 9017.
Burgess, A.W., Metcalf, D. 1980. J. Cancer26, 647.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable