ABS2090

Anti-ICDH

serum, from rabbit

• Sinónimos: Isocitrate dehydrogenase, ICDH (NADP), IDH
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: serum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: E. coli, Legionella pneumophila
• envase: antibody small pack of 25 μL
• técnicas: western blot: suitable
• isotipo: IgG
• Nº de acceso GenBank: GAN25950.1
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Isocitrate dehydrogenase [NADP] (also known as IDH, ICDH) catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate. In eukaryotes there are at least three isozymes of IDH: two are located in the mitochondrial matrix (one NAD+-dependent, the other NADP+-dependent), while the third one (also NADP+-dependent) is cytoplasmic. In E.coli the activity of a NADP+-dependent ICDH is controlled by the phosphorylation of a serine residue and the phosphorylated form of ICDH is completely inactivated. It has been reported that if Ser113 is replaced by alanine, cysteine, threonine, or tyrosine there is some loss of enzymatic activity. However, substitution by aspartic acid completely abolishes its activity. ICDH contains three domains: a large alpha + beta domain, a small alpha/beta domain and an alpha/beta clasp-like domain involving both subunits. (Ref.: Hurley, JH et al (1989). Proc. Natl. Acad. Sci. USA. 86 (22); 8635-8639).

Especificidad

Expected to react with most eubacteria.

This polyclonal antibody detects isocitrate dehydrogenase in E. coli and Legionella pneumophila.

Inmunógeno

Epitope: unknown

His-tagged full length recombinant isocitrate dehydrogenase from Legionella pneumophila, strain Lp02.

Aplicación

Anti-ICDH, Cat. No. ABS2090, is a rabbit polyclonal antibody that detects Isocitrate dehydrogenase [NADP]. It has been tested for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected ICDH in 20 µL Legionella cell lysate.

Western Blotting Analysis: A representative lot detected ICDH in E. Coli cell lysate (Xu, L., et. al. (2010). PLoS Pathog. 6(3):e1000822; Lu, C., et. al. (2016). Sci Signal. 9(412):ra11).

Calidad

Evaluated by Western Blotting in E.Coli cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected ICDH in 20 µL E.Coli cell lysate.

Descripción de destino

~45 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Forma física

Rabbit polyclonal antiserum with 0.05% sodium azide.

Unpurified

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to lot specific datasheet.

Información legal

GenBank is a registered trademark of United States Department of Health and Human Services

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable