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AB9208

Sigma-Aldrich

Anti-DARPP-32 Antibody, phosphoThr75

Chemicon®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
clone:
polyclonal
application:
WB
species reactivity:
human, mouse
technique(s):
western blot: suitable
citations:
1

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... PPP1R1B(84152)

Specificity

DARPP-32, phosphoThr75.

Immunogen

Epitope: phosphoThr75
Synthetic peptide from human/mouse DARPP-32, phosphoThr75.

Application

Detect DARPP-32 using this Anti-DARPP-32 Antibody, phosphoThr75 validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Reward & Addiction

Signaling Neuroscience
Western blot: 1:1,000. The antibody recognizes the ~34 kD DARPP-32 protein phosphorylated at Thr75 protein in rat caudate lysate prepared from caudate slices that had been incubated in the absence and presence of okadaic acid. The labeling by the antibody to DARPP-32 Thr75 is markedly increased by the okadaic acid treatment.

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Liquid in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg per mL BSA with 50% glycerol.

Storage and Stability

Maintain at -20°C to -80°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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