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69390-M

Sigma-Aldrich

Bacteriophage CE6 - Novagen

λ phage for delivery of T7 RNA polymerase

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About This Item

Código UNSPSC:
41106602
NACRES:
NA.54
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nivel de calidad

100

Formulario

liquid

fabricante / nombre comercial

Novagen®

condiciones de almacenamiento

OK to freeze
avoid repeated freeze/thaw cycles

temp. de almacenamiento

−70°C

Descripción general

λ phage for delivery of T7 RNA polymerase
Bacteriophage CE6 is a recombinant lambda phage containing the T7 RNA polymerase gene (Studier 1986). The polymerase gene is cloned into the int gene, such that it is transcribed from the pL and pI promoters of the phage during infection. λCE6 can be used to provide a source of T7 RNA polymerase to susceptible host cells carrying T7 expression plasmids. In general, it is used when a target gene is so toxic that the plasmid cannot be maintained in DE3 lysogenic hosts, or with alternative hosts that are not DE3 lysogens. The phage has λcI857 immunity and the Sam7 mutation, which requires supF hosts for lytic growth (neither lytic growth nor different immunity are required for T7 RNA polymerase synthesis). It is provided as a high titer lysate and includes host strains LE392 (rK12 mK12+) and ED8739 (rK12 mK12) as glycerol stocks. The 10-ml size is enough to induce up to 100 ml culture.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Componentes

•0.2 or 10 mlCE6 Lysate

•0.2 mlLE392 glycerol stock

•0.2 mlED8739 glycerol stock

Advertencia

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Otras notas

Studier, F.W. and Moffatt, B.A. 1986 J. Mol. Biol. 189, 113.

Información legal

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Questions

  1. Is it necessary to include maltose?

    1 answer

    1. Yes, It is recommended to add maltose as it increases the lambda phage receptors on the surface of the E. coli cells, which is beneficial for the mutagenesis process.

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