1.20589
GranuCult® PEMBA (Polymyxin Pyruvate Egg yolk Mannitol Bromothymolblue Agar) (base)
ISO 21871, Selective Medium for B. cereus
Sinónimos:
PEMBA agar (base)
About This Item
Productos recomendados
agency
ISO 21871
Quality Level
sterility
non-sterile; Not processed or packaged aseptically
form
medium granules
manufacturer/tradename
GranuCult®
pH range
7.0 - 7.4 (25 °C)
application(s)
food and beverages
pathogen testing
storage temp.
15-25°C
suitability
selective and differential for bacteria: Bacillus Cereus
General description
B. cereus grow as turquoise to peacock blue colonies, since the medium is slightly alkalized by its metabolites (bromothymol blue reaction). The yolk reaction (by lecithinase) also leads to a precipitate surrounding the colony, which appears in the color of the agar or similar to the colony. Growth and lecithinase activity are often so strong that the precipitation is already evident before a colony is visible.
Bacillus cereus is identified by colony morphology, color, and egg yolk hydrolysis. Microscopic examination of the cell and spore morphology and the formation of intracellular lipid globules in vegetative cells is a rapid method of confirmation.
Mannitol-positive bacteria form yellow colonies with a yellow zone in the surrounding medium.
Application
Principle
Footnote
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
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Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
Certificados de análisis (COA)
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