1.10338

Fractogel^® EMD Chelate (M)

purified by immobilized metal affinity chromatography (IMAC)

• Sinónimos: Fractogel^® EMD Chelate (M)
• Código UNSPSC: 41115711

Propiedades

• ligand: iminodiacetic acid
• Nivel de calidad: 400
• descripción: for metal affinity chromatography (IMAC), suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
• esterilidad: sterile (Caustic Stable)
• Formulario: resin
• purificado por: immobilized metal affinity chromatography (IMAC)
• fabricante / nombre comercial: Fractogel^®
• Parámetros: 8 bar max. pressure; ~240 cm/hr flow rate
• grupo activo de la matriz: methacrylate
• Ancho: 40-90 μm
• capacidad: ~60 mg binding capacity( lysozyme/ml of resin)
• temperatura de transición: flash point 35 °C (calculated)
• aplicaciones: recombinant protein
• técnica de separación: affinity
• temp. de almacenamiento: 2-30°C

Descripción

Descripción general

Fractogel^® EMD Chelate combines our tentacle structure with iminodiacetic acid as the metal chelating ligand

Características y beneficios

Fractogel^® EMD Chelate (M) enables users to:

• Efficiently purify histidine-tagged recombinant proteins
• Coordinate metal ions to non-bonding electron pairs of the amino acid side chains and separate peptides
• Ensure timely and successful startup and operation
• Minimize hydrophobic interactions due to the tentacles technology, resulting in superior selectivity and recovery
• Handle multiple CIP and packing cycles with the chemically and physically robust bead, leading to a longer resin lifetime

Envase

• 1.10338.0100: Fractogel^® EMD Chelate (M) Resin 100ml
• 1.10338.0010: Fractogel^® EMD Chelate (M) Resin 10 ml
• 1.10338.0500: Fractogel^® EMD Chelate (M) Resin 500 ml
• 1.10338.5000: Fractogel^® EMD Chelate (M) Resin 5 L

Plastic bottle

Nota de análisis

Appearance: Milky tubid suspension, free from impurities (foreigen particles)
Microscopic evaluation: Uniform spherical particles, no agglomerates, no fines
Cerium: ≤ 10 µg/g
Copper-(II)-binding capacity: 60 - 100 µmol/ml
Extractable matter (water): ≤ 0.05 %
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Functional test: passes test
Functional test: Separation of chymotrypsinogen A and myoglobin

Información legal

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS02
• Palabra de señalización: Warning
• Frases de peligro: H226
• Consejos de prudencia: P210 - P233 - P240 - P241 - P242 - P243
• Clasificaciones de peligro: Flam. Liq. 3
• Código de clase de almacenamiento: 3 - Flammable liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): 95.0 °F
• Punto de inflamabilidad (°C): 35 °C