06-157
Anti-RasGAP Antibody
Upstate®, from rabbit
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, rat, mouse, avian
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TSR3(115939)
Specificity
GAP
Immunogen
Trp E fusion corresponding to residues 171-448 of human RasGAP
Application
Anti-RasGAP Antibody is an antibody against RasGAP for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
Quality
Western Immunoblot Analysis: This antibody, at 0.5 and 2.0 μg/ml, recognized the 120 kDa GAP protein in a RIPA lysate of human Raji lymphocyte cells, lysates of rat L6 myoblasts and mouse Wehi leukemia cells.
Immunoprecipitation: 5 μg of this antibody immunoprecipitated the 120kDa GAP protein from a RIPA lysate of human Raji lymphocyte cells.
Immunoprecipitation: 5 μg of this antibody immunoprecipitated the 120kDa GAP protein from a RIPA lysate of human Raji lymphocyte cells.
Target description
120kDa
Linkage
Replaces: 04-577; 04-1039
Physical form
200 μg of protein-A purified IgG. Lyophilized from 200 μl of 0.1 M Tris-HCl, 0.15 M NaCl, and 0.05% Na Azide.
Format: Purified
Protein A chromatography
Storage and Stability
Lyophilized: 2 years at -20°C; Rehydrated: 6 months at -20°C Rehydrate in 200 μl of water.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
Certificados de análisis (COA)
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S J Ruff et al.
The Journal of biological chemistry, 272(2), 1263-1267 (1997-01-10)
The intraperitoneal injection of a vanadate/H2O2 mixture (peroxovanadate) into mice resulted within minutes in the appearance of numerous tyrosine-phosphorylated proteins in the liver and kidney. These effects are presumably due to the inhibition of phosphotyrosine phosphatase activity. Three of the
F Authier et al.
The Biochemical journal, 319 ( Pt 1), 149-158 (1996-10-01)
We have investigated the biosynthesis, subcellular location and expression of insulin-degrading enzyme (IDE). a type-I peroxisomal protease, in semi-permeabilized hepatoma cells using pulse-chase experiments, non-denaturing immunoprecipitation protocols and Northern-blot analyses. In HcpG2 cell lysates prepared from cells radiolabelled with Tran[35S]-label
A conserved region of c-Ha-Ras is required for efficient GTPase stimulation by GTPase activating protein but not neurofibromin
Yoder-Hill, J., et al
The Journal of Biological Chemistry, 270, 27615-27621 (1995)
S J Taylor et al.
The Journal of biological chemistry, 270(17), 10120-10124 (1995-04-28)
The c-Src tyrosine kinase phosphorylates and binds to a 68-kDa RNA-binding protein in mitotic cells. We have examined the mechanism and functional consequence of the interaction of c-Src with this protein, Sam 68 (Src associated in mitosis, 68 kDa). In
P J Owen-Lynch et al.
The Journal of biological chemistry, 270(11), 5956-5962 (1995-03-17)
A temperature-sensitive mutant of the v-Abl protein has previously been shown to exhibit tyrosine protein kinase activity in Interleukin 3 (IL-3)-dependent IC.DP cells grown at the permissive temperature (32 degrees C) but not at the restrictive temperature (39 degrees C).
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