Saltar al contenido
Merck
Todas las fotos(1)

Documentos clave

Ver Toda la documentación

330002S

Avanti

E06 mAb-biotinylated

E06 Mouse Monoclonal Antibody (IgM), Biotinylated Anti-(Oxidized Phospholipid), 1X TBS (Tris Buffered Saline) with 1%BSA

Sinónimos:

E06 mAb-biotinylated, T15 antibody

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Código UNSPSC:
12352203
NACRES:
NA.45
En este momento no podemos mostrarle ni los precios ni la disponibilidad

forma del anticuerpo

purified from hybridoma cell culture

tipo de anticuerpo

primary antibodies

Ensayo

>95%

Formulario

solution

envase

pkg of 1 × 0.125 mL (polypropylene tube with screw cap (330002S-25ug))

fabricante / nombre comercial

Avanti Research™ - A Croda Brand 330002S

concentración

0.2 mg/mL (330002S-25ug)

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Descripción general

Avanti now provides high purity E06 from an animal-free culture of hybridoma cells. The E06 antibody was developed by Dr. Joseph Witztum at the University of California, San Diego using apolipoprotein E knock-out mice fed a high-fat diet for seven months.

Aplicación

E06 mAb-biotinylated has been used in immunodetection.[1] It may be used in immunohistochemistry,[2] western blot analysis and in in vivo imaging with magnetic resonance imaging (MRI) techniques.
The E06-Biotin is primarily used for sandwich ELISA determination of ox-LDL in serum or plasma samples. It can also be used in direct ELISA, competitive (IHC) and Western Blot analysis. For use in direct ELISA, make a 4 μg/mL working solution of 12 μg E06-Biotin in 3 mL of 1% BSA in PBS (1:62.5 dilution). Make a serial (1:1) dilution of this working solution to titrate the antibody. For competitive ELISA make a 2.5 μg/mL working solution (1:100 dilution).

Acciones bioquímicas o fisiológicas

The E06 antibody is a murine monoclonal IgM natural antibody that binds to oxidized phospholipid and reacts with oxidized Low-density lipoprotein (LDL),[3] oxidized High-density lipoprotein (HDL) and protein covalently modified by oxidized phospholipid. This antibody has been used to probe atherosclerotic plaque in rabbits, monkeys,[2] mice, zebrafish and humans. It also binds to apoptotic cells, but not viable cells.

Envase

0.5 mL Polypropylene Tube with Screw Cap (330002S-25ug)

Información legal

Avanti Research is a trademark of Avanti Polar Lipids, LLC

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documentos section.

Si necesita más asistencia, póngase en contacto con Atención al cliente

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice. Demonstration of epitopes of oxidized low density lipoprotein in human plasma.
Palinski W, et al.
The Journal of Clinical Investigation, 98(3), 800-814 (1996)
Increased plasma oxidized phospholipid: apolipoprotein B-100 ratio with concomitant depletion of oxidized phospholipids from atherosclerotic lesions after dietary lipid-lowering: a potential biomarker of early atherosclerosis regression
Tsimikas S, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology, 27(1), 175-181 (2007)
Impact of inhibition of the autophagy-lysosomal pathway on biomolecules carbonylation and proteome regulation in rat cardiac cells
Coliva G, et al.
Redox Biology, 23, 101123-101123 (2019)

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico