T5050

Transforming Growth Factor-β1 from porcine platelets

≥97% (SDS-PAGE), recombinant, powder, suitable for cell culture

• Synonym(s): TGF-β1
• MDL number: MFCD00166089
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• product name: Transforming Growth Factor-β1 from porcine platelets, TGF-β1, powder, suitable for cell culture
• biological source: Porcine (Platelets)
• Quality Level: 100
• Assay: ≥97% (SDS-PAGE)
• form: powder
• potency: 0.01-0.1 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• mol wt: protein 25 kDa
• packaging: pkg of 1 μg; pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg
• UniProt accession no.: P07200
• storage temp.: −20°C
• Gene Information: pig ... TGFB1(397078)

Description

General description

The TGFB (transforming growth factor-β) gene is mapped to pig (Sus scrofa) chromosome between 6q11-q21. In mammals, TGFβ is present in three isoforms (TGFβ1, TGFβ2 and TGFβ3), of which TGFβ1 is predominant.

Application

Transforming Growth Factor-β1 from porcine platelets has been used in chondrogenic differentiation. It has also been used in immobilization process.

Biochem/physiol Actions

Transforming growth factor-β1 (TGF-β) is a multifunctional polypeptide growth factor. TGF-β mediates cell proliferation, differentiation and migration. Important cellular functions such as inflammation, extracellular matrix synthesis, epithelial restoration and developmental processes are also controlled by TGF-β. TGF-β controls the regulation of enamel mineralization in porcine. TGF-β is associated with the development of idiopathic pulmonary fibrosis (a chronic lung disease). TGF-β promotes epithelial-mesenchymal transition (EMT) and contributes to proliferation and metastasis of cancer. The TGF-β induced EMT is observed in kidney fibrosis. TGF-β is stimulates regeneration of intestinal tissue.

Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.

Physical form

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% trifluoroacetic acid containing 0.05 mg bovine serum albumin per μg cytokine.

Analysis Note

The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves