T4427
Terminal Transferase from calf thymus
buffered aqueous glycerol solution
Synonym(s):
TdT, Terminal Deoxynucleotidyl Transferase
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About This Item
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grade
Molecular Biology
for molecular biology
form
buffered aqueous glycerol solution
mol wt
60 kDa
concentration
>5000 U/mL
UniProt accession no.
foreign activity
Exonuclease and endonuclease, free
storage temp.
−20°C
Gene Information
cow ... DNTT(281120)
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General description
Bovine terminal transferase (TdT) is a primer-dependent polymerase that catalyzes the addition of deoxynucleotides to the 3′-OH terminus of DNA molecules with the release of inorganic phosphate. TdT reacts preferentially with either single-stranded DNA molecules or double-stranded-DNA with 3′ overhangs, but procedures have been developed to label blunt ends or 3′-recessive ends. In a reaction mixture, the divalent ion (Co2+, Mn2+, Mg2+) will influence purine and pyrimidine polymerization rate. Activities of TdT are also affected by the bases (dATP, dCTP, dGTP and dTTP) present.
Application
Suitable for:
- Addition of homopolymers to vectors, inserts and cDNA for cloning
- Labeling the 3′-end of double- and single-stranded DNA with non-radioactive or radioactive labels
- Carrying out in vitro mutagenesis by adding single nucleotides to DNA
- Use in TUNEL assays
Components
Terminal transferase is supplied as a solution of 50 mM potassium phosphate (pH 7.4), 1 mM 2-mercaptoethanol and 50% glycerol (v/v).
Unit Definition
One unit will incorporate 1 nanomole of dATP into acid-precipitable material in one hour at 37 °C using d(pT)6 as primer.
Analysis Note
Activity assay uses 200 mM potassium cacodylate, pH 7.2, 4 mM MgCl2, 1 mM 2-mercaptoethanol, 1 mM 3H-dATP, 70 μM d(pT)6, 37 °C.
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Description
Pricing
Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution
Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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C P Tu et al.
Gene, 10(2), 177-183 (1980-07-01)
Cordycepin-5'-triphosphate (3'-deoxyadenosine-5'-triphosphate) can be incorporated into the 3'-ends of DNA fragments using terminal deoxynucleotidyl transferase from calf thymus (Bollum, 1974). Because cordycepin-5'-monophosphate lacks a 3'-OH group, only a single residue is incorporated. Furthermore, DNA molecules that contain cordycepin-5'-monophosphate at their
Marcel Hollenstein et al.
Bioorganic & medicinal chemistry letters, 22(13), 4428-4430 (2012-05-29)
Pyrene-deoxynucleoside triphosphates (dPTPs), varying by the positioning of the aromatic system, were synthesized. Their ability to function as substrates for the Klenow fragment of Escherichia coli DNA polymerase I and the TdT polymerase was assessed. The dPTPs are all equally
Yuan Zhang et al.
Cellular immunology, 274(1-2), 19-25 (2012-04-03)
Secondary rearrangements of the T cell receptor (TCR) represent a genetic correction mechanism which changes T cell specificity by re-activating V(D)J recombination in peripheral T cells. Murine T-cell hybridoma A1.1 was employed to investigate whether antigenic stimulation induced re-expression of
Botao Zhao et al.
Acta biochimica et biophysica Sinica, 44(2), 129-135 (2011-12-23)
MicroRNAs (miRNAs) constitute a critically important class of non-translated, small RNAs, which post-transcriptionally regulate gene expression via one of the multiple mechanisms. To profile miRNA expression, microarrays have been extensively applied to the high-throughput detection of miRNAs. Here, we described
Tadashi Terada
International journal of clinical and experimental pathology, 5(2), 167-170 (2012-03-09)
Although the definition of precursor T lymphoblastic lymphoma (T-LBL) is based only on histopathology, most cases cannot be diagnosed only by HE sections. Since 95% of T-LBL expresses TdT and TdT is expressed only in lymphoblasts, immunohistochemical demonstration of TdT
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