S1265
Anti-Sheep IgG (whole molecule) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
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About This Item
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biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
indirect ELISA: 1:50,000-1:100,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other rabbit serum proteins. Antiserum is determined to be immunospecific for sheep IgG by immunoelectrophoresis (IEP) versus sheep IgG and normal sheep serum. Identity and purity of the antibody is established by immunoelectrophoresis (IEP). Electrophoresis of the product followed by diffusion versus the anti-rabbit IgG and the anti-rabbit whole serum result in single arcs of precipitation in the γ region.
Specificity
Binds all sheep Igs
Immunogen
purified sheep IgG
Application
Anti-Sheep IgG (whole molecule) antibody produced in rabbit has been used in:
- Enzyme-linked immunosorbent assay (ELISA)
- Immunoprecipitation
- labelling electrodes in immunosensor systems
Biochem/physiol Actions
Digestion of IgG by papain generates fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallisable (fc). IgG antibody has enormous therapeutic potential and the Fc is involved in the development of therapeutic antibody.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as preservative.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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