Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.
Application
Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.
Components
10x PBS contains:
30mM sodium phosphate
1.5 M sodium chloride
10.5 mM potassium phosphate
Preparation Note
Dilute 10x PBS to a 1x working solution using molecular biology grade water.
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