MAK164
Intracellular Hydrogen Peroxide Assay
sufficient for 200 fluorometric tests (green fluorescence)
Synonym(s):
Hydrogen Peroxide Detection in Cells
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
Recommended Products
usage
sufficient for 200 fluorometric tests (green fluorescence)
detection method
fluorometric
relevant disease(s)
aging/geriatric diseases; neurological disorders; pulmonary disorders; orthopedic diseases; cardiovascular diseases
storage temp.
−20°C
Related Categories
General description
Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.
Application
Intracellular hydrogen peroxide assay kit has been used to measure intracellular hydrogen peroxide levels.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
This kit is suitable for the detection of hydrogen peroxide in living cells and a variety of samples such as cell extracts and liquids.
Principle
The Intracellular Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in living cells and in a variety of other samples such as cellular extracts. This kit utilizes a unique cell-permeable sensor that generates a fluorescent product (λex = 490/λem = 520 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader, flow cytometer, or fluorescent microscope.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Robert B Rosenblatt et al.
Theranostics, 11(2), 602-613 (2021-01-05)
Mechanical forces from non-ablative pulsed focused ultrasound (pFUS) generate pro-inflammatory tumor microenvironments (TME), marked by increased cytokines, chemokines, and trophic factors, as well as immune cell infiltration and reduced tumor growth. pFUS also causes DNA damage within tumors, which is
Maqusood Ahamed et al.
International journal of nanomedicine, 16, 89-104 (2021-01-16)
Therapeutic selectivity and drug resistance are critical issues in cancer therapy. Currently, zinc oxide nanoparticles (ZnO NPs) hold considerable promise to tackle this problem due to their tunable physicochemical properties. This work was designed to prepare SnO2-doped ZnO NPs/reduced graphene
Emily A Teslow et al.
Molecular oncology, 13(4), 894-908 (2019-01-13)
Obesity is a risk factor for triple-negative breast cancer (TNBC) incidence and poor outcomes, but the underlying molecular biology remains unknown. We previously identified in TNBC cell cultures that expression of epigenetic reader methyl-CpG-binding domain protein 2 (MBD2), specifically the
Maqusood Ahamed et al.
International journal of environmental research and public health, 17(21) (2020-11-12)
Combined exposure of single-walled carbon nanotubes (SWCNTs) and trace metal lead (Pb) in ambient air is unavoidable. Most of the previous studies on the toxicity of SWCNTs and Pb have been conducted individually. There is a scarcity of information on
Qing You et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(17), 1903341-1903341 (2020-10-01)
Multifunctional nanoplatforms for imaging-guided synergistic antitumor treatment are highly desirable in biomedical applications. However, anticancer treatment is largely affected by the pre-existing hypoxic tumor microenvironment (TME), which not only causes the resistance of the tumors to photodynamic therapy (PDT), but
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service