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MAK022

Sigma-Aldrich

Calcium Colorimetric Assay

sufficient for 250 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 250 colorimetric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric

relevant disease(s)

neurological disorders; hematological disorder

storage temp.

2-8°C

General description

Calcium, the most abundant mineral in the human body, is a crucial intracellular element that is responsible for regulating many physiological and pathological processes.[1] Calcium is found in either the free ion form or in bound complexes, for example the calcium phosphate and calcium carbonate complexes that make up bone tissue. Numerous physiological processes, including muscle contraction, cell adhesion, hormones/ neurotransmitters release, glycogen metabolism, cell proliferation/differentiation, blood clotting, nerve or synapthetic impulse transmission, and structural support of the skeleton are regulated by calcium signaling.[2] Defects in the integrity of cell-specific calcium signaling systems may be associated with certain human diseases.

Application

Calcium Colorimetric Assay kit has been used to measure calcium concentration in hippocampal samples and MC3T3-E1 mouse osteoblast cell line, which were cultured in osteogenic induction medium.[3][4]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for use with cell and tissue culture supernatants, urine, plasma, serum, fecal material, media, and other biological fluids.

Principle

In this assay, the calcium ion concentration is determined by the chromogenic complex formed between calcium ions and o-cresolphthalein, which is measured at 575 nm and is proportional to the concentration of calcium ions present. The linear range of detection for this kit is between 0.4-2.0 μg.

replaced by

Product No.
Description
Pricing

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H315,H318,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8L23K03800
8B25K03800
8H02K03800
7G13K03800
7C25K03800

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Questions

1–5 of 5 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Could you provide the Kd (binding affinity) for calcium and clarify if the kit measures all calcium or just free/unbound calcium?

    1 answer

    1. The kit uses the chromogenic complex (λ = 575 nm) formed between calcium ions and 0-cresolphthalein, specifically measuring free calcium and not the bound calcium. However, there is no available information regarding the Kd (binding affinity).

      Helpful?

  4. For the analysis of the standard curve, is it supposed to result in a linear curve or plotted as a polynomial? 

    1 answer

    1. The standard curve for product MAK022 should be fitted using linear regression. The x-axis will be the 0 (assay blank), 0.4, 0.8, 1.2, 1.6, and 2.0 μg/well standards with the OD 575 nm on the y-axis for each of the respective standards after the standard blank subtraction.

      Helpful?

  5. Hello, just wanted to confirm if this assay detects the free Ca2+ only and not the bound Calcium, correct?

    1 answer

    1. Yes, this kit measures only free calcium and not bound calcium. The kit utilizes the chromogenic complex formed between calcium ions and 0-cresolphthalein.

      Helpful?

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