E4531
Anti-ELKS antibody, Mouse monoclonal
clone ELKS-30, purified from hybridoma cell culture
Synonym(s):
Anti-KIAA1081
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ELKS-30, monoclonal
form
buffered aqueous solution
mol wt
antigen ~120 kDa
species reactivity
rat, chicken, bovine, human, mouse
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.5-1 μg/mL using HeLa cell extract
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ERC1(23085)
mouse ... Erc1(111173)
Related Categories
General description
ELKS/RAB6-interacting/CAST family member 1 is a protein encoded by the ERC1 gene in humans. It encodes for a synaptic factor and is found in the smallest region of overlap.
Monoclonal Anti-ELKS (mouse IgG2a isotype) is derived from the hybridoma ELKS-30 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human ELKS. ELKS, also known as KIAA1081, is an essential regulatory subunit of the IKK complex. It was named ELKS since 44.1% of the sequence is composed of glutamic acid (E), leucine (L), lysine (K) and serine (S) residues. The protein contains 948 amino acids with nine -helical coiled-coil domains including periodic heptad repeats that predict dimer formation. Its highest expression is in heart, placenta, lung, brain, thyroid and testis.
Application
Monoclonal Anti-ELKS antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- fluorescence immunohistochemistry
- immunocytochemistry
- immunoblotting
Biochem/physiol Actions
ELKS/RAB6-interacting/CAST family member 1 is a protein encoded by the ERC1 gene in humans. It encodes for a synaptic factor and is found in the smallest region of overlap. ERC1 encodes a regulator of neurotransmitter release and acts as the best gene candidate contributing to this phenotype as well as to the autism spectrum disorder ASD. ERC1a (an isoform of ERC1) is associated with other proteins for effective migration and tumor cell invasion by stabilizing the activity at the cell front. Depletion of any of the associated protein negatively affects invasion, migration on extracellular matrix, lamellipodial persistence and the internalization of active integrin β1 receptors needed for adhesion turnover at the front of the cell.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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12p13.33 microdeletion including ELKS/ERC1, a new locus associated with childhood apraxia of speech.
Julien Thevenon et al.
European journal of human genetics : EJHG, 21(1), 82-88 (2012-06-21)
Speech sound disorders are heterogeneous conditions, and sporadic and familial cases have been described. However, monogenic inheritance explains only a small proportion of such disorders, in particular in cases with childhood apraxia of speech (CAS). Deletions of <5 Mb involving
Fusion of a novel gene, ELKS, to RET due to translocation t (10; 12)(q11; p13) in a papillary thyroid carcinoma
Nakata T, et al.
Genes Chromosomes Cancer, 25(2), 97-103 (1999)
Jie Chen et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 31(2), 512-525 (2011-01-14)
Synapse formation requires the organization of presynaptic active zones, the synaptic vesicle release sites, in precise apposition to postsynaptic neurotransmitter receptor clusters; however, the molecular mechanisms responsible for these processes remain unclear. Here, we show that P/Q-type and N-type voltage-dependent
Wei Wang et al.
The Journal of biological chemistry, 290(21), 13372-13385 (2015-04-12)
DNA damage-induced NF-κB activation plays a critical role in regulating cellular response to genotoxic stress. However, the molecular mechanisms controlling the magnitude and duration of this genotoxic NF-κB signaling cascade are poorly understood. We recently demonstrated that genotoxic NF-κB activation
Dillon Jevon et al.
eLife, 11 (2022-05-14)
A developing understanding suggests that spatial compartmentalisation in pancreatic β cells is critical in controlling insulin secretion. To investigate the mechanisms, we have developed live-cell subcellular imaging methods using the mouse organotypic pancreatic slice. We demonstrate that the organotypic pancreatic
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