The synthetic peptide corresponds to amino acid residues 413-426 of rat ET-A (Accession P26684) and has multiple isoforms. There are multiple glycosylation and phosphorylation sites (Accession P26684). After performing a western blot, you will observe multiple bands, with sizes approximately around 48 KDa, 28 KDa, and so on. For further details on the protein size and its different isoforms, please visit the UniProt website and refer to the following link: UniProt - Accession P26684.
E3651
Anti-Endothelin Receptor A antibody produced in rabbit
affinity isolated antibody, lyophilized powder
Synonym(s):
Anti-ET-A, Anti-ET1-Specific Endothelin Receptor
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About This Item
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
human, pig, chicken, bovine
technique(s)
immunohistochemistry: 1:100 using rat brain sections or rat lung paraffin embedded sections
western blot: 1:200 using rat brain membranes and ECL
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... EDNRA(1909)
rat ... Ednra(24326)
Specificity
The epitope is highly conserved in human, swine, bovine and chicken ET-A.
Immunogen
synthetic peptide corresponding to amino acids 413-426 of rat ET-A (with additional N-terminal cysteine).
Application
Anti-Endothelin Receptor A antibody produced in rabbit is suitable for immunohistochemistry at a dilution of 1:100 using rat brain sections or rat lung paraffin embedded sections and western blotting at a dilution of 1:200 using rat brain membranes and ECL.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
Biochem/physiol Actions
Endothelin receptor type A (ETA, ETAR) is a human G protein-coupled receptor that mediates vasoconstriction of blood vessels. It is involved in tumor cell invasion, survival and metastasis. ETAR mediates the promotion of ET-1 on colon cancer cell survival, invasion and MMP-2 expression by a PI3K-mediated mechanism. It plays a critical role in colon cancer metastasis.
Physical form
Lyophilized from a solution of phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Shaolin Nie et al.
Molecular carcinogenesis, 53 Suppl 1, E85-E91 (2013-07-03)
The endothelin (ET)-1/endothelin A receptor (ETAR) axis is reportedly involved in tumor cell invasion, survival, and metastasis. However, the role of ETAR in colon cancer metastasis and the underlying mechanisms have not been defined. In the present study, we assessed
S Hori et al.
Endocrinology, 130(4), 1885-1895 (1992-04-01)
Endothelins (ETs) are very potent vasoconstrictive peptides and have diverse functions in both vascular and nonvascular tissues. This investigation concerns the tissue distribution and cellular localization of rat mRNAs encoding two different subtypes of ET receptors (ETA and ETB). We
Shaoqing He et al.
Investigative ophthalmology & visual science, 56(10), 6144-6161 (2015-09-24)
A growing body of evidence suggests that the vasoactive peptides endothelins (ETs) and their receptors (primarily the ETB receptor) are contributors to neurodegeneration in glaucoma. However, actions of ETs in retinal ganglion cells (RGCs) are not fully understood. The purpose
Kwangkyu Lee et al.
Protein expression and purification, 84(1), 14-18 (2012-05-09)
Human endothelin receptor type A (ET(A)) is a G-protein coupled receptor that mediates vasoconstriction of blood vessels. To determine the structural characteristics and signaling mechanism of ET(A), we have expressed recombinant ET(A) as a fusion protein with p9 envelope protein
H Y Lin et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(8), 3185-3189 (1991-04-15)
By screening a cDNA library derived from the A10 rat vascular smooth muscle cell line for functional expression in COS cells, we have isolated a high-affinity receptor for endothelin 1 (Kd = 476 pM) and endothelin 2. The affinity of
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