EJG

93051803, bovine adrenal gland, Endothelial

• UNSPSC Code: 41106514

Properties

• product name: EJG, 93051803
• biological source: bovine adrenal gland
• growth mode: Adherent
• karyotype: Not specified
• morphology: Endothelial
• products: Factor VIII
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Bovine adrenal medulla endothelial

Cell Line Description

The bovine cell line was established from collagenase-dissociated, adrenal medulla tissue. The cells display a capillary endothelium morphology with relatively large cell bodies and produce factor VIII:C. Tests for BVDV have shown that the cell line is producing both detectable BVDV antigens and infectious BVDV.

Application

Blood clotting studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Passage cells every 5 to 7 days.

Other Notes

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