A9231
Acridine Orange solution
2% in H2O
Synonym(s):
3,6-Acridinediamine, N,N,N′,N′-tetramethyl-, monohydrochloride, 3,6-Bis(dimethylamino)acridine hydrochloride
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All Photos(2)
About This Item
Empirical Formula (Hill Notation):
C17H19N3 · HCl
CAS Number:
Molecular Weight:
301.81
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Recommended Products
form
liquid
Quality Level
shelf life
18 mo.
concentration
2% in H2O
technique(s)
microbe id | staining: suitable
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
SMILES string
N(C)(C)c1cc2nc3c(cc2cc1)ccc(c3)N(C)C
InChI
1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3
InChI key
DPKHZNPWBDQZCN-UHFFFAOYSA-N
General description
Acridine Orange (C.l. 46005) is an acridine dye with a ring structure and overall small planar conjugated system.
Application
Acridine orange is a DNA intercalating dye that is a recommended DNA stain for flow cytometry. It differentially stains double-stranded and single-stranded nucleic acids.
It also has the following applications-
It also has the following applications-
- staining of RNA and acid mucins
- detecting apoptosis
- staining blood smears to diagnose malaria
- identifying and enumerating granulocytes in fish
- staining eukaryotic cells to demonstrate low pH structures
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Matthew P Harris et al.
PLoS genetics, 4(10), e1000206-e1000206 (2008-10-04)
The genetic basis of the development and variation of adult form of vertebrates is not well understood. To address this problem, we performed a mutant screen to identify genes essential for the formation of adult skeletal structures of the zebrafish.
Marko Gerić et al.
Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering, 47(11), 1521-1527 (2012-06-19)
The present study aimed to characterize chemical properties of clay bricks containing 20 % of sewage sludge. After detection of potentially hazardous metals, we simulated precipitation exposure of such material to determine the amount of heavy metals that could leach
Donald P Evenson
Methods in molecular biology (Clifton, N.J.), 927, 147-164 (2012-09-21)
The SCSA(®) is the pioneering assay for the detection of damaged sperm DNA and altered proteins in sperm nuclei via flow cytometry of acridine orange (AO) stained sperm. The SCSA(®) is considered to be the most precise and repeatable test
T V Kuznetzova et al.
Genetika, 48(4), 451-456 (2012-06-27)
A novel phenomenon of unusual selective acridine orange (AO) staining ofpericentromeric heterochromatin regions (HRs) in chromosomal preparations from tissue with known spontaneous mitotic activity (chorionic villi, placenta, embryonic tissues, bone marrow, and testes), as well as embryonic stem cells, is
Dolar Pak et al.
Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes, 48(6), 512-515 (2013-03-05)
In this study we assessed the use of acridine orange as an alternative to optical density to quantify the growth of Lactobacillus bulgaricus ATCC 7517. The growth of bacteria in Lactobacillus de Man Rogosa Sharpe (MRS) medium was measured by
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