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64958

Sigma-Aldrich

2-Methoxy-2,4-diphenyl-3(2H)-furanone

suitable for fluorescence, ≥98.0% (HPLC)

Synonym(s):

2,4-Diphenyl-2-methoxy-3(2H)-furanone, MDPF

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About This Item

Empirical Formula (Hill Notation):
C17H14O3
CAS Number:
50632-57-0
Molecular Weight:
266.29
Beilstein:
1288529
MDL number:
MFCD00036834
UNSPSC Code:
12352108
PubChem Substance ID:
329760016
NACRES:
NA.32

Assay

≥98.0% (HPLC)

form

solid

fluorescence

λex 384 nm; λem 472 nm in acetonitrile (after derivatization with hexylamine)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

COC1(OC=C(C1=O)c2ccccc2)c3ccccc3

InChI

1S/C17H14O3/c1-19-17(14-10-6-3-7-11-14)16(18)15(12-20-17)13-8-4-2-5-9-13/h2-12H,1H3

InChI key

BLWINLJDTOJSRU-UHFFFAOYSA-N

Application

2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) is used as a fluorescence reagent to derivatize primary and secondary amines on molecules such as proteins. MDPF derivatized molecules can be detected during analytical and separation procedures including chromatography, electrophoresis and zymography.

Other Notes

Reagent for the derivatization of primary and secondary amines for HPLC; highly fluorescent derivatives are formed; Pre-column derivatization of amines; Fluorescent labeling of proteins before SDS-PAGE

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S K Williams et al.
Journal of cellular physiology, 120(2), 157-162 (1984-08-01)
The transport of proteins across continuous capillary endothelium is believed to be mediated by micropinocytic vesicles which shuttle molecules between the lumenal and abluminal plasma membrane. We have studied the ability of capillary endothelial cells isolated from rat epididymal fat
R C Schaeffer et al.
International journal of microcirculation, clinical and experimental, 11(2), 181-201 (1992-05-11)
We studied the macromolecular size-selective transport characteristics of polycarbonate (PC) filters with defined pore radius (rp; 15,000 to 400 A) as well as 2,000 A rp PC filter-bovine pulmonary artery endothelial cell (EC) monolayer sandwich under zero hydrostatic pressure conditions
F J Alba et al.
Electrophoresis, 19(14), 2407-2411 (1998-11-20)
The fluorogenic dye 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) has been used for the detection of total protein patterns on polyvinylidene difluoride (PVDF) membranes. Fluorescent staining of protein bands on membranes with this covalent dye is completed in 20 min. Wet membranes are translucent
B W Falk et al.
Analytical biochemistry, 144(2), 537-541 (1985-02-01)
Fluorescent labeling of proteins was found to be a very sensitive and reliable alternative to conventional methods for monitoring proteins on Western blots. Proteins were labeled with 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) before SDS-PAGE. After electrophoresis and subsequent electro-blotting the fluorescent-labeled proteins were
S. Stein
Pept. Neurobiol., 9-9 (1977)
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