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Merck

52403

Sigma-Aldrich

Anti-Mouse IgG−Abberior® STAR 580 antibody produced in goat

for STED application

Synonym(s):

Abberior® STAR 580-Anti-Mouse-IgG antibody produced in goat

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

goat

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

availability

available only in USA and Canada

concentration

~1 mg/mL

fluorescence

λex 587 nm; λem 607 nm in PBS, pH 7.4

storage temp.

−20°C

General description

Abberior STAR 580 was developed for STED and confocal microscopy in the orange spectral region. Abberior STAR 580 is highly photostable and bright orange fluorescent dye. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. It can be very effectively excited with excitation wavelength between 550 - 600 nm. Abberior STAR 580 can substitute dyes like Cy3, Atto 590 or Alexa Fluor 568. For STED microscopy, Abberior STAR 580 can be used most efficiently with a STED Laser wavelength between 750 - 800 nm. Abberior STAR 580 is the ideal partner for Abberior STAR RED or Abberior STAR 635P to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 587 (PBS pH 7.4), 584 (H2O), 584 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 85 000 (PBS pH 7.4), 85 000 (H2O), 95 000 (MeOH)
Correction Factor, CF260 = ε260max: 0,17 (PBS pH 7.4)
Correction Factor, CF280 = ε280max: 0,17 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 607 (PBS pH 7.4), 607 (H2O), 604 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,5 (PBS pH 7.4)

Application

Anti-Mouse IgG-Abberior® STAR 580 antibody produced in goat has been used for STED (stimulated emission depletion) microscopy in CA3 (Cornus Ammonis) sections.[1]

Suitability

Designed and tested for fluorescent super-resolution microscopy

Analysis Note

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
Cy3 is a trademark of Cytiva
abberior is a registered trademark of Abberior GmbH

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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