Skip to Content
Merck
All Photos(1)

Key Documents

TMS-012

Sigma-Aldrich

Dulbecco's Phosphate Buffered Saline

1X, liquid, w/o Ca++ & Mg++, Endotoxin-Free

Synonym(s):

DPBS, PBS, Phosphate buffered saline

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352207
eCl@ss:
32160405
NACRES:
NA.75
Pricing and availability is not currently available.

Product Name

Endotoxin-Free Dulbecco′s PBS (1X) (w/o Ca++ & Mg++), Cell Culture

Quality Level

300

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | mammalian: suitable

Related Categories

General description

Endotoxin-Free Dulbecco’s PBS (1X) (w/o Ca++ & Mg++) is sterile filtered through a 0.1 micron filter and is considered endotoxin-free (<0.005 EU/ml).

Application

Endotoxin-Free Dulbecco′s PBS (1X) (w/o Ca++ & Mg++) has been used as a wash buffer during:
  • the isolation of colonic lamina propria cells
  • teratoma formation by induced pluripotent stem cells (iPSCs)
  • production of elastic porous injectable (EPI) biomaterial from the bulk scaffolds

Quality

Appearance: Clear Liquid/No Particulates
Sterility Testing: Negative for Growth
pH: 7.0-7.2
Osmolality: 260-305 mOsm/kg
Endotoxin: <0.005 EU/mL

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

ADCC Protocol using BioIVT Cryopreserved NK Effector Cells

Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.

Alzheimer’s In A Dish™: 3D Neural Stem Cell Models of Alzheimer's Disease

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

Questions

  1. Is this made with Bacteriostatic water? I need to reconstitute an enzyme with endotoxin free PBS but the enzyme is inhibited by bacteriostatic water.

    1 answer

    1. The method of preparation is considered proprietary. However, the term 'bacteriostatic' typically implies the presence of benzyl alcohol. No benzyl alcohol is present in the water used to prepare this product.
      The composition is as follows:
      0.2 g/L KCL
      0.2 g/L KH2PO4
      8.0 g/L NaCl
      1.15 g/L Na2HPO4 (anhydrous)

      With the pH adjusted to pH 7.0 to 7.1 using HCl.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service