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1.15938

Sigma-Aldrich

Hematoxylin monohydrate (C.I.75290)

for microscopy Certistain®

Synonym(s):

Hematoxylin monohydrate (C.I.75290)

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About This Item

Empirical Formula (Hill Notation):
C16H14O6 · xH2O
Molecular Weight:
302.28 (anhydrous basis)
UNSPSC Code:
41116130
EC Index Number:
208-237-3
NACRES:
NA.21

Quality Level

400

form

solid

composition

Dye, ≥95% spectrophotometric assay

IVD

for in vitro diagnostic use

mp

140 °C (Elimination of water of crystallisation)

solubility

slightly soluble at 20 °C

bulk density

500 kg/m3

εmax

155-165 at 290-292 nm in acetonitrile at 0.05 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

Related Categories

General description

Hämatoxylin-Monohydrat (C.I. 75290) for microscopy Certistain®, is a dye for the specific staining of DNA in the nuclei. It is used in cytology and histology. Nuclei are stained dark blue, dark violet to black. Hematoxylins are mixed with trivalent positively charged metal salts and build up the so-called hematoxylin lakes, used for the selective staining of nuclei (DNA). Certistain® is the quality group for seleceted high quality dyes that fulfill all quality requirements for IVD and CE registration. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 95 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (acetonitrile): 290 - 292 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.05 g/l; acetonitrile): 155 - 165
Haematein (spectrophotometrically): ≤ 0.1 %
Water (according to Karl Fischer): 5 - 6 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Certificates of Analysis (COA)

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