R1153

RNase B Glycoprotein Standard from bovine pancreas

≥90% (SDS-PAGE), lyophilized powder

• Synonym(s): Ribonuclease B from bovine pancreas, RNase B
• CAS Number: 9001-99-4
• EC Number: 232-646-6
• MDL number: MFCD00132184
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• product name: RNase B Glycoprotein Standard from bovine pancreas, Proteomics Grade
• grade: Proteomics Grade
• Quality Level: 200
• Assay: ≥90% (SDS-PAGE)
• form: lyophilized powder
• storage temp.: −20°C
• InChI: 1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
• InChI key: CQOVPNPJLQNMDC-UHFFFAOYSA-N

Description

Application

RNase B is a preferred substrate with PNGase F for demonstration of N-linked deglycosylation using SDS-PAGE or MALDI-MS. The activity of PNGase F is routinely assayed using RNase B by monitoring the pronounced mobility shift in 12% gels after deglycosylation. Proteomics Grade RNase B has been used as a source of N-glycans following enzymatic digestions and subsequent purification. It has also been used as a glycoprotein standard.

Other Notes

Bovine pancreatic RNase B is a glycoprotein that contains only N-linked glycans. It is a globular protein composed of a single domain that occurs naturally as a lesser component in mixture along with ribonuclease A (RNase A), which is the non-glycosylated core form. RNase B contains a single glycosylation site at Asn³⁴ at which from five to nine mannose residues are attached to the chitobiose core, i.e. Man_5-9GlcNAc₂. Due to the heterogeneity in the glycosylation at Asn³⁴, RNase B exists as five glycosylated varients, with a molecular weight of approx. 15 kDa.

Quality

RNAse B Glycoprotein Standard has been highly purified to remove contaminating RNase A.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves