This product is an ammonium sulfate suspension. This is an enzyme, and batch-specific Certificates of Analysis report the activity of the enzyme. Most customers use it as an active enzyme. Please reach out to local technical support for clarification on using the inactivated form of the enzyme.
R1003
Ribonuclease T1 from Aspergillus oryzae
ammonium sulfate suspension, 300,000-600,000 units/mg protein
Synonym(s):
Guanyloribonuclease, Ribonucleate 3′-guanylo-oligonucleotidohydrolase
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About This Item
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biological source
Aspergillus sp. (Aspergillus oryzae)
Quality Level
form
ammonium sulfate suspension
specific activity
300,000-600,000 units/mg protein
mol wt
11068 by amino acid sequence
technique(s)
cell based assay: suitable
suitability
suitable for separating native or denatured proteins, or nucleic acids
application(s)
cell analysis
storage temp.
2-8°C
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Application
Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies [1].
Biochem/physiol Actions
Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3′-phosphate group of a guanidine ribonucleotide and 5′-hydroxyl of the adjacent nucleotide. The initial product is a 2′:3′ cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3′-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3′-GMP and oligonucleotides with a 3′-GMP terminal group.
Unit Definition
One unit will produce acid soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 15 min at pH 7.5 at 37°C, in a reaction volume of 1.0 mL. Substrate: Yeast RNA.
Physical form
Suspension in 2.8 M (NH4)2SO4 solution
Analysis Note
Protein determined by E1%/280
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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This product was in powder form before and is now in liquid form. Do you still have to inactivate 20 min at 80°C before use? Thank you very much
1 answer-
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Is it possible to store this product frozen at -20°C?
1 answer-
Storing ammonium sulfate suspensions of proteins frozen can lead to the removal of ammonium sulfate from the protein crystal structure. This exposes the protein to a high amount of ammonium sulfate, potentially causing denaturation due to high salt content. Consequently, the enzyme activity may decrease, as may have occurred in this instance. It is recommended that the customer purchase a new bottle and store it at 2-8°C.
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