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N2889

Sigma-Aldrich

Neutral Red Solution (0.33%)

3.3 g/L in DPBS, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Neutral Red solution, 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Toluylene red

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About This Item

Empirical Formula (Hill Notation):
C15H17ClN4
CAS Number:
Molecular Weight:
288.78
MDL number:
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

product line

BioReagent

form

solution

concentration

3.3 g/L in DPBS

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

SMILES string

[Cl-].Cc1cc2N=C3C=C\C(C=C3Nc2cc1N)=[N+](/C)C

InChI

1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H

InChI key

PGSADBUBUOPOJS-UHFFFAOYSA-N

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General description

Neutral Red is a cationic dye that is used for nuclear counterstaining in biological research. It also serves as a pH indicator in analytical laboratories. On decomposition, neutral red dye releases carbon monoxide (CO), carbon dioxide (CO2), nitrogen oxides, and hydrogen chlorides. Therefore, this dye is considered toxic and is recommended to remove before discharge into receiving streams.

Application

Neutral Red Solution (0.33%) has been to stain viable cells and measure cell viability. It has also been used as a component of culture medium for enzymatic digestion of ovarian medulla tissue to extract follicles.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Sewar Alkhashrom et al.
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Nuclear egress is an essential process in the replication of human cytomegalovirus (HCMV), as it enables the migration of newly formed viral capsids from the nucleus into the cytoplasm. Inhibition of the HCMV core nuclear egress complex (core NEC), composed
William J Melvin et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(38) (2021-09-05)
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Silvia Tampucci et al.
Molecules (Basel, Switzerland), 24(9) (2019-05-12)
In this work, hybrid compounds 1-4 obtained by conjugation of the non-steroidal anti-inflammatory drug diclofenac, with natural molecules endowed with antioxidant and antiproliferative activity were prepared. The antiproliferative activity of these hybrids was evaluated on immortalized human keratinocyte (HaCaT) cells
Sheikh Md Rajiuddin et al.
Food and environmental virology, 12(4), 295-309 (2020-09-05)
The leading causes of foodborne viral disease outbreaks are human norovirus and hepatitis A virus (HAV). Their environmental persistence enables contamination of kitchen surfaces and crops often consumed raw, such as berries. Many decontamination procedures are inefficient and unsuitable for
Nadia M DiNunno et al.
Nature communications, 11(1), 4953-4953 (2020-10-04)
Zika virus (ZIKV) is an emerging mosquito borne flavivirus and a major public health concern causing severe disease. Due to the presence of a lipid membrane and structural heterogeneity, attaining an atomic resolution structure is challenging, but important to understand

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