L7275

LB Broth (Lennox)

Tablet microbial growth medium

• Synonym(s): Lennox broth , Luria Bertani Broth
• UNSPSC Code: 12352200
• NACRES: NA.85

Properties

• grade: for molecular biology
• sterility: non-sterile
• form: tablet
• technique(s): microbiological culture: suitable
• application(s): food and beverages; microbiology
• storage temp.: room temp
• suitability: nonselective for Escherichia coli; nonselective for coliforms

Description

General description

Dehydrated Luria-Bertani (LB) broth is called the Lennox LB, which has amino acids as carbon source.

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

Application

LB Broth (Lennox) has been used for culturing Escherichia coli and bacterial artificial chromosomes (BACs).

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^®.

Features and Benefits

Lennox LB tablets provide:

• Convenient small package to eliminate weighing
• Fast preparation – no stirring required

Components

10 g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
2.2 g/L inert binding agents

Reconstitution

Add each 1.1g tablet in 48.3mL water (no stirring required). Autoclave for 20 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Legal Information

Zeocin is a registered trademark of Cayla Sarl

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable