L2898
Lysostaphin from Staphylococcus staphylolyticus
aseptically filled
Synonym(s):
Glycyl-glycine Endopeptidase
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
sterility
aseptically filled
Quality Level
form
powder
specific activity
>500 units/mg protein
mol wt
25 kDa
composition
Protein, 40-70% biuret
antibiotic activity spectrum
Gram-positive bacteria
Mode of action
cell wall synthesis | interferes
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
General description
Chemical structure: peptide
Lysostaphin is mainly liberated as a proenzyme. It is highly stable at pH 4 and temperature 5°C.
Biochem/physiol Actions
Lysostaphin exhibit a lytic action against Staphylococcus aureus. It possesses several functions of three major enzymes, such as, glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acteyl muramyl-L-alanine amidase. Lysostaphin can be used to treat antibiotic-resistant staphylococcal infections. Lysostaphin being an antistaphylococcal agent, can be used as a preservative in the food industry and in clinical labs for rapid screening.
Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5
pH Optimum for activity: ~7.5
Unit Definition
One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 °C in a 6.0 ml reaction mixture.
Preparation Note
Prepared from L 7386
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Lysostaphin: an antistaphylococcal agent
Kumar J K
Applied Microbiology and Biotechnology, 80(4), 555-561 (2008)
Lysostaphin and clarithromycin: a promising combination for the eradication of Staphylococcus aureus biofilms.
A Aguinaga et al.
International journal of antimicrobial agents, 37(6), 585-587 (2011-04-19)
Mohammad Mehdi Feizabadi et al.
Journal of microbiological methods, 84(1), 144-146 (2010-11-17)
A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Staphylococcus aureus and enterococci to reduce the cost of using lysostaphin. This protocol reduces the expenses of PFGE typing of S. aureus and enterococci as
Huanli Liu et al.
Applied and environmental microbiology, 77(24), 8500-8508 (2011-10-11)
Lactobacillus species are a predominant member of the vaginal microflora and are critical in maintaining an acidic vaginal environment thought to contribute to the prevention of a number of urogenital diseases. However, during menstruation the pH of the vaginal environment
Hong Wu et al.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 27(11), 1623-1630 (2012-03-08)
The purpose of this paper is to establish sulfhydryl site-directed PEGylation method for lysostaphin and to evaluate effects of mutagenesis and modification of amino acid residue within putative linker on enzyme activity. On the basis of structural analysis of lysostaphin
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service