C0897
Citrate Lyase from Klebsiella pneumoniae
lyophilized powder, ≥0.20 unit/mg solid
Synonym(s):
Citrate oxaloacetate lyase
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About This Item
Recommended Products
form
lyophilized powder
Quality Level
specific activity
≥0.20 unit/mg solid
mol wt
>5 kDa
storage temp.
2-8°C
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Application
Citrate Lyase from Klebsiella pneumoniae has been used in the digestion of low molecular weight human milk fraction (>5 kDa referred to as 5kF).
Biochem/physiol Actions
Citrate lyase is found in several microorganisms and catalyzes the first step of citrate degradation, forming acetate and oxaloacetate. The enzyme contains 3 polypeptide subunits, α-subunit (a transferase), β-subunit (acyl lyase) and γ-subunit (acyl-carrier protein).
Unit Definition
One unit will convert 1.0 μmole of citrate to oxalacetate per min at pH 7.6 at 25 °C.
Physical form
Lyophilized powder containing bovine serum albumin, sucrose, and MgSO4
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Citrate lyase [EC 4.1.3.6; citrate oxaloacetate-lyase (pro-3S-CH2-COO--leads to acetate)] from Klebsiella aerogenes has been dissociated with urea; the three different subunits, alpha-chain (molecular weight congruent to 54,000), beta-chain (molecular weight congruent to 32,000), and gamma-chain (acyl carrier protein; molecular weight
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In this paper, we describe two open reading frames coding for a NAD-dependent malic enzyme (mae) and a putative regulatory protein (clyR) found in the upstream region of citCDEFG of Leuconostoc mesenteroides subsp. cremoris 195. The transcriptional analysis of the
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