C0794
Chitosanase from Streptomyces sp.
buffered aqueous glycerol solution, ≥15 units/mg protein (E1%)
Synonym(s):
Chitosan N-acetylglucosaminohydrolase
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
form
buffered aqueous glycerol solution
Quality Level
specific activity
≥15 units/mg protein (E1%)
concentration
≥0.5 mg/mL
solubility
0.1 M acetate buffer, pH 5.5: soluble
storage temp.
−20°C
Related Categories
Application
Chitosanase from Sigma has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan.
Biochem/physiol Actions
Chitosanase catalyzes the endohydrolysis of β-1,4-linkages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN linkage in partially acetylated chitosan.
Unit Definition
One unit will liberate 1.0 μmole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.5 at 37 °C.
Physical form
Solution in 50% glycerol containing 50 mM sodium acetate
Preparation Note
Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Aretz, W., et al.
FEMS Microbiology Letters, 65, 31-31 (1989)
Anna Konieczna-Molenda et al.
Carbohydrate research, 343(18), 3117-3119 (2008-10-01)
Illumination with white linearly polarized light (WLPL) stimulated chitinase and chitosanase in their degradation of chitin and chitosan, respectively. Enzymes were illuminated at room temperature in separate vessels, then admixed in reactors containing polysaccharides. Hydrolysis of chitosan to glucosamine followed
Tahira Naz et al.
Frontiers in nutrition, 8, 756218-756218 (2021-11-02)
Canthaxanthin is a reddish-orange xanthophyll with strong antioxidant activity and higher bioavailability than carotenes, primarily used in food, cosmetics, aquaculture, and pharmaceutical industries. The spiking market for natural canthaxanthin promoted researchers toward genetic engineering of heterologous hosts for canthaxanthin production.
A new reaction for colorimetric determination of carbohydrates.
M Lever
Analytical biochemistry, 47(1), 273-279 (1972-05-01)
Boucher, I., et al.
The Journal of Biological Chemistry, 270, 31-31 (1995)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service