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Merck

95284

Sigma-Aldrich

Water

for molecular biology, suitable for FISH

Synonym(s):

H2O

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About This Item

Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
MDL number:
UNSPSC Code:
12191602
PubChem Substance ID:
NACRES:
NA.31

Pricing and availability is not currently available.

product name

Water, for molecular biology, sterile filtered

grade

for molecular biology

sterility

sterile-filtered

form

liquid

quality

sterile filtered

shelf life

limited shelf life, expiry date on the label

technique(s)

FISH: suitable

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.34 (lit.)

pH

5-7

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This Item
W1754W3500270733
Water for molecular biology, sterile filtered

95284

Water

Water PCR Reagent

W1754

Water

Water sterile-filtered, BioReagent, suitable for cell culture

W3500

Water

Water suitable for HPLC

270733

Water

technique(s)

FISH: suitable

technique(s)

PCR: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

HPLC: suitable

form

liquid

form

liquid

form

liquid

form

liquid

mp

0 °C (lit.)

mp

0 °C (lit.)

mp

0 °C (lit.)

mp

0 °C (lit.)

pH

5-7

pH

5-7

pH

5-7

pH

6.0-8.0 (25 °C)

bp

100 °C (lit.)

bp

100 °C (lit.)

bp

100 °C (lit.)

bp

100 °C (lit.)

General description

Molecular biology grade water is sterile filtered and ready to use, free from DNase, RNase, phosphatase, and protease contamination.

Application

Water, sterile filtered has been used:

  • for cloning and plasmid construction.[1]
  • for restriction digestion of PCR amplicons in polysaccharide A (PSA) restriction method.[2]
  • in RNA isolation.[3][4][5]
  • fluorescence in situ hybridization (FISH).[6]
  • to dilute the samples of CVF (cervicovaginal fluid) and menstrual extracts for testing specificity through reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of mRNA. [7]
  • for DNA quantification using quantitative polymerase chain reaction (qPCR).[8]

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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A guide to transient expression of membrane proteins in HEK-293 cells for functional characterization
Ooi A, et al.
Frontiers in Physiology, 7, 300-300 (2016)
Registered report: Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukemia
Fung J, et al.
eLife, 4, e08997-e08997 (2015)
L1 retrotransposon expression in circulating tumor cells
Papasotiriou I, et al.
Testing, 12(2), e0171466-e0171466 (2017)
Andreas K Brödel et al.
Nature protocols, 12(9), 1830-1843 (2017-08-11)
Directed evolution is a powerful tool to improve the characteristics of biomolecules. Here we present a protocol for the intracellular evolution of proteins with distinct differences and advantages in comparison with established techniques. These include the ability to select for
Philippe Robert et al.
Investigative radiology, 50(8), 473-480 (2015-06-25)
To prospectively compare in healthy rats the effect of multiple injections of macrocyclic (gadoterate meglumine) and linear (gadodiamide) gadolinium-based contrast agents (GBCAs) on T1-weighted signal intensity in the deep cerebellar nuclei (DCN), including the dentate nucleus. Healthy rats (n =

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