28616

Atto 425-Biotin

BioReagent, suitable for fluorescence, ≥95.0% (HPCE)

• Synonym(s): Biotin-Atto 425
• Empirical Formula (Hill Notation): C₃₇H₅₃N₅O₇S
• Molecular Weight: 711.91
• MDL number: MFCD04974818
• UNSPSC Code: 12352108
• eCl@ss: 34058011
• PubChem Substance ID: 57648315
• NACRES: NA.32

Properties

• product line: BioReagent
• Quality Level: 100
• Assay: ≥95.0% (HPCE)
• form: powder
• manufacturer/tradename: ATTO-TEC GmbH
• fluorescence: λ_ex 437 nm; λ_em 483 nm in 0.1 M phosphate pH 7.0
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(=O)NCCCCCNC(=O)CCCCC4SCC5NC(=O)NC45)c3cc2OC1=O
• InChI: 1S/C37H53N5O7S/c1-5-48-34(45)26-19-24-18-25-23(2)21-37(3,4)42(28(25)20-29(24)49-35(26)46)17-11-14-32(44)39-16-10-6-9-15-38-31(43)13-8-7-12-30-33-27(22-50-30)40-36(47)41-33/h18-20,23,27,30,33H,5-17,21-22H2,1-4H3,(H,38,43)(H,39,44)(H2,40,41,47)
• InChI key: LPVYZZMUDCZDQO-UHFFFAOYSA-N

Description

General description

Atto 425 is a new label with high quantum yield (0.90) as well as sufficient stoke′s shift (excitation maximum 436 nm, emission maximum 484 nm). It is optimized for excitation with argon laser, and is characterized by high photostability. It does nearly not show cis-trans-isomerization, which limits brightness and reproducibility for many other dyes.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 425 biotin is suitable for fluorescence labeling of avidin/streptavidin conjugated molecules.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves