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63052

Sigma-Aldrich

Magnesium acetate solution

BioUltra, for molecular biology, ~1 M in H2O

Synonym(s):

Magnesium acetate anhydrous, Mg(OAc)2

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About This Item

Linear Formula:
(CH3COO)2Mg
CAS Number:
Molecular Weight:
142.39
Beilstein:
3692530
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

form

liquid

concentration

~1 M in H2O

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.358

pH

5.0-7.0 (25 °C)

cation traces

Al: ≤5 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤5 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mn: ≤5 mg/kg
Mo: ≤1 mg/kg
Na: ≤20 mg/kg
Ni: ≤2 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.04
λ: 280 nm Amax: 0.02

SMILES string

CC(=O)O[Mg]OC(C)=O

InChI

1S/2C2H4O2.Mg/c2*1-2(3)4;/h2*1H3,(H,3,4);/q;;+2/p-2

InChI key

UEGPKNKPLBYCNK-UHFFFAOYSA-L

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Application

Magnesium acetate is used in molecular biology applications such as batch in vitro transcription of RNA and the crystallization of transcription factor:DNA complexes and proteins via the sitting-drop vapor-diffusion method.
Magnesium acetate solution has been used:
  • to supplement Midkin fused to the biotin ligase BioID2 (MK-BioID2) conditioned medium for in-tube biotinylation assay
  • as a component of lysis buffer and amplified RNA (aRNA) fragmentation buffer to perform aRNA fragmentation
  • as a component of lysis buffer and DamID buffer for sequencing zygote samples

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Surya Agung Priyambada et al.
Virus research, 238, 101-109 (2017-06-25)
Hepatitis B virus (HBV) is the smallest partially double-stranded DNA virus known to infect humans. Worldwide, more than 50% of hepatocellular carcinoma (HCC) cases are related to chronic Hepatitis B. Development of HCC from normal liver cells is characterized by
Cinthia R Millan et al.
Nucleic acids research, 45(14), 8378-8391 (2017-06-24)
Trypanosoma brucei, the causative agent of sleeping sickness (Human African Trypanosomiasis, HAT), contains a kinetoplast with the mitochondrial DNA (kDNA), comprising of >70% AT base pairs. This has prompted studies of drugs interacting with AT-rich DNA, such as the N-phenylbenzamide
Linda E Franken et al.
Nature communications, 8(1), 722-722 (2017-09-30)
Bacteria downregulate their ribosomal activity through dimerization of 70S ribosomes, yielding inactive 100S complexes. In Escherichia coli, dimerization is mediated by the hibernation promotion factor (HPF) and ribosome modulation factor. Here we report the cryo-electron microscopy study on 100S ribosomes
Yoshitaka Matsuo et al.
Nature communications, 8(1), 159-159 (2017-08-02)
Translation arrest by polybasic sequences induces ribosome stalling, and the arrest product is degraded by the ribosome-mediated quality control (RQC) system. Here we report that ubiquitination of the 40S ribosomal protein uS10 by the E3 ubiquitin ligase Hel2 (or RQT1)
Tomohide Saio et al.
eLife, 7 (2018-05-02)
Molecular chaperones alter the folding properties of cellular proteins via mechanisms that are not well understood. Here, we show that Trigger Factor (TF), an ATP-independent chaperone, exerts strikingly contrasting effects on the folding of non-native proteins as it transitions between

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