15604

Urea

puriss., meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%, 99.0-101.0% (calc. on dry substance)

• Synonym(s): Carbamide, Carbonyldiamide
• Linear Formula: NH₂CONH₂
• CAS Number: 57-13-6
• Molecular Weight: 60.06
• Beilstein: 635724
• EC Number: 200-315-5
• MDL number: MFCD00008022
• UNSPSC Code: 12352100
• PubChem Substance ID: 329750941
• NACRES: NA.21

Properties

• grade: puriss.
• Quality Level: 200
• Assay: 99.0-100.5%; 99.0-101.0% (calc. on dry substance)
• form: solid
• quality: meets analytical specification of Ph. Eur., BP, USP
• impurities: alkalic reac. substances, complies; ≤0.001% heavy metals (as Pb); ≤0.04% insoluble in ethanol; ≤0.05% ammonium (NH₄); ≤0.1% biuret(carbamylurea)
• ign. residue: ≤0.1% (as SO₄)
• loss: ≤1.0% loss on drying, 105 °C, 1 h
• mp: 132-135 °C (lit.); 132-135 °C
• solubility: H₂O: soluble 480 g/L at 20 °C
• density: 1.335 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤10 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Fe: ≤0.5 mg/kg
• suitability: in accordance for appearance of solution; in accordance for identity
• functional group: amine
• SMILES string: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N

Description

General description

Urea is a colorless, odorless solid. It has prism-like crystals. It forms insoluble nitrate salts with nitric acid and oxalate salts with oxalic acid. On reaction with alkanes or substituted alkanes, it affords crystal-lattice inclusion compounds.
Urea can be prepared from ammonia and carbon-dioxide, via Haber-Bosch process. It can also be used to prepare urea-formaldehyde resins, via condensation reaction.

Application

Urea has been used in the following studies:

• Synthesis of SnO₂ nanoparticles.
• Synthesis of urethanes, ureides and semicarbazides.
• Denaturation of protein isolated from Saccharomyces cerevisiae Y15696 strain
• Protein electrophoretic studies.

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves