10197734001

Glutamate Dehydrogenase (GlDH)

from beef liver

• Synonym(s): DNA marker
• Enzyme Commission number: 1.4.1.3 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: bovine liver
• Quality Level: 100
• form: lyophilized
• specific activity: 10 U/mg; ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)
• packaging: pkg of 3,000 U
• manufacturer/tradename: Roche
• concentration: ≥10-20 % (w/w)
• technique(s): activity assay: suitable
• color: white
• optimum pH: 8
• solubility: water: 20 mg/mL, colorless
• suitability: suitable for UV spectrophotometry and general use
• UniProt accession no.: P00366
• application(s): life science and biopharma
• foreign activity: ADH <0.00500%; LDH <0.00500%; MDH <0.00500%
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: bovine ... GLUD1(281785)

Description

General description

Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.

GDH (Glutamate dehydrogenase) is a hexamer of 449 residues and is located in the mitochondria. It acts as a branch-point enzyme between amino acid oxidation and urea production. L-glutamate:NAD(P)⁺ oxidoreductase is involved in deamination.

Application

Glutamate dehydrogenase has been used to measure residual ammonium by enzymatic analysis during fermentation by wine yeast. It used as a component of cofactor recycling systems for NAD(P) and NAD(P)H.

Quality

Contaminants: <0.005% ADH, LDH, and MDH, each

Sequence

The smallest active structure of GIDH (310,000 to 350,000 ) is a hexamer subunit of 55,400 D. This hexamer reversibly self-assembles forming a polymer with up to eight hexamers (2,200 kD).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash