Traditional DNA/RNA isolation kits can be used to prepare genomic material for sequencing (RNA-seq, scRNA-seq, WGS, CHIP-seq etc). For some applications with large organoids, tissue specific DNA/RNA extraction kits are recommended. TRI Reagent (T9424) can be added to the dissociated organoids before RNA extraction and can be stored at -80 °C until future use.
SCM304
3dGRO® Human Colon Organoid Expansion Medium
Complete serum-free organoid media for the expansion and long-term 3D culture of human intestinal colon organoids.
Synonym(s):
Colon Organoid Culture Media, Intestinal Organoid Culture Media
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.71
Pricing and availability is not currently available.
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form
liquid
packaging
pkg of 50 mL
technique(s)
cell culture | stem cell: suitable
osmolality
350-375 mOsm
application(s)
cell analysis
General description
3dGRO® Human Colon Organoid Expansion Medium is a complete ready-to-use serum-free medium for the expansion and long-term culture of human colonic organoids. The medium has been validated for use with 3dGRO® Human iPSC Derived Colon Organoids (Cat. No. SCC300). Colon organoids propagated in the medium express colon-specific markers including the posterior hindgut marker CDX2, a-carbonic anydrase II (CA-II), a-carbonic anhydrase IV (CA-IV), and goblet cell markers Mucin-2 and Mucin-5B.
Application
Complete serum-free organoid media for the expansion and long-term 3D culture of human intestinal colon organoids.
Research Category:
Research Category:
- Cell Culture
- Stem Cell Research
Quality
Appearance (Color): Clear/No Particlulates (Red Liquid)
Osmolality: 350-375 mOsm
pH: 7.0 – 7.4
Sterility Tested: No Growth/Pass
Endotoxin: <2 EU/mL
Mycoplasma: Negative
Functional Assay: Thaw and culture of human colon organoids for 2 passages.
Osmolality: 350-375 mOsm
pH: 7.0 – 7.4
Sterility Tested: No Growth/Pass
Endotoxin: <2 EU/mL
Mycoplasma: Negative
Functional Assay: Thaw and culture of human colon organoids for 2 passages.
Storage and Stability
Upon receipt, store at −20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly. Use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at −20°C until the expiry date.
Legal Information
3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug screening and compound toxicity testing.
Protocols
A rapid in vitro assay for CFTR function, the forskolin-induced swelling protocol uses human colon organoids, which can be derived from cystic fibrosis patient tissue.
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.
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How Do you Extract RNA/DNA from Organoids in Cultured in Matrigel?
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How are organoids cultured?
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Once isolated, organoids are cultured in specialized media embedded in a rich extracellular matrix (ECM) basement membrane extract hydrogel such as Growth Factor Reduced Matrigel. Media is replenished every other day and cells are passaged once per week (7-12 days) before cells become too large or necrotic. Cells can be passaged in either small clump fragments or as single cells using mechanical dissociation/enzyme-free passaging reagents. Depending on organoid type and confluency, split ratios of roughly 1:3-1:4 can be used when passaging organoids. For sensitive organoid types, ROCKi (Y27632) (SCM075) can be added during passaging to help in promoting cell viability.
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How do you stain organoids with antibodies?
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Organoids can be stained with antibodies for immunocytochemical (ICC)/Immunohistochemical (IHC) analysis using either whole-mount or paraffin embedding/sectioning techniques. It is critical to use antibodies prequalified for organoid analysis.
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