MAB365
Anti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG
clone 192-IgG, Chemicon®, from mouse
Synonym(s):
CD271 antigen, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, low affinity nerve growth factor receptor, nerve growth factor receptor, nerve growth factor receptor (TNFR superfamily, member 16), p75 IC
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
192-IgG, monoclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NGFR(4804)
General description
The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells.
Specificity
Reacts with rat p75 protein.
Specific for the rat low affinity Nerve Growth Factor-Receptor (p75), does not react with human or mouse p75.
Immunogen
Epitope: Extracellular
n-octoglucoside stabilized proteins containing p75 receptor, which were isolated from plasma membranes of PC-12 cells (Chandler et al., 1984).
Application
Anti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG is an antibody against Nerve Growth Factor Receptor for use in IC, IH, IP, FUNC & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neurochemistry & Neurotrophins
Western Blot:
1-5 µg/mL (Non-reducing conditions only) was used on a previous lot.
Inhitibition Assay:
Inhibition on NGF binding {Chandler, 1984}.
Immunoprecipitation:
5 µg/mL, 0.5% triton X-100 buffer; PC12 positive control was used on a previous lot.
Immunohistochemistry:
1-5 µg/mL on rat spinal chord, of a previous lot was used. Other fixatives and methods can also be used.
1-5 µg/mL (Non-reducing conditions only) was used on a previous lot.
Inhitibition Assay:
Inhibition on NGF binding {Chandler, 1984}.
Immunoprecipitation:
5 µg/mL, 0.5% triton X-100 buffer; PC12 positive control was used on a previous lot.
Immunohistochemistry:
1-5 µg/mL on rat spinal chord, of a previous lot was used. Other fixatives and methods can also be used.
Quality
Immunohistochemistry:
1-5 µg/mL on rat , of a previous lot, (spinal chord, see protocol below); other fixatives and methods can also be used.
1-5 µg/mL on rat , of a previous lot, (spinal chord, see protocol below); other fixatives and methods can also be used.
Target description
75 kDa
Linkage
Replaces: 04-1111
Physical form
Format: Purified
Mouse monoclonal IgG1 in 0.02M Phosphate buffer, pH7.6, 0.25M NaCl with 0.1% sodium azide
Protein A purified
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
Western Blot: NGF-differentiated PC12 cell lysate.
Immunohistochemistry: Rat brain tissue.
Western Blot: NGF-differentiated PC12 cell lysate.
Immunohistochemistry: Rat brain tissue.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Tissue engineering the monosynaptic circuit of the stretch reflex arc with co-culture of embryonic motoneurons and proprioceptive sensory neurons.
Guo, X; Ayala, JE; Gonzalez, M; Stancescu, M; Lambert, S; Hickman, JJ
Biomaterials null
Audrey P Le et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(2), 703-712 (2012-01-13)
The neurotrophin nerve growth factor (NGF) regulates neuronal growth, differentiation, and survival during development. However, the precursor of NGF, proNGF, is a potent apoptotic ligand for the p75 neurotrophin receptor (p75(NTR))-sortilin complex. The mechanisms that regulate cleavage of proNGF, therefore
Abel Torres-Espín et al.
PloS one, 8(10), e76141-e76141 (2013-10-23)
Transplantation of bone marrow derived mesenchymal stromal cells (MSC) or olfactory ensheathing cells (OEC) have demonstrated beneficial effects after spinal cord injury (SCI), providing tissue protection and improving the functional recovery. However, the changes induced by these cells after their
Shayri G Greenwood et al.
eNeuro, 5(1) (2018-01-20)
Neurotrophins play critical roles in the survival, maintenance and death of neurons. In particular, proneurotrophins have been shown to mediate cell death following brain injury induced by status epilepticus (SE) in rats. Previous studies have shown that pilocarpine-induced seizures lead
Marta Volosin et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(39), 9870-9879 (2008-09-26)
Seizure-induced damage elicits a loss of hippocampal neurons mediated to a great extent by the p75 neurotrophin receptor (NTR). Proneurotrophins, which are potent apoptosis-inducing ligands for p75(NTR), were increased in the hippocampus, particularly in astrocytes, by pilocarpine-induced seizures; and infusion
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