G1512
β-Glucuronidase from Helix pomatia
Type H-5, lyophilized powder, ≥400,000 units/g solid
Synonym(s):
β-D-Glucuronide glucuronosohydrolase
Sign Into View Organizational & Contract Pricing
All Photos(5)
About This Item
Recommended Products
type
Type H-5
form
lyophilized powder
specific activity
≥400,000 units/g solid
secondary activity
≤40,000 units/g solid sulfatase
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
General description
β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Application
β-Glucuronidase from Helix pomatia has been used:
- in the enzymatic treatment of urine samples for liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis
- in the quercetin extraction procedure from pig or rat tissues for high performance liquid chromatography (HPLC) analysis
- in the enzymatic deconjugation step in urine and plasma sample preparation for gas chromatography-mass spectrometry analysis (GC-MS)
β-glucuronidase was used to hydrolyze conjugated glucuronide and sulfate metabolites.
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Biochem/physiol Actions
β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Quality
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Linkage
A further purification of G0876 by gel permeation chromatography.
Unit Definition
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Measurement of intact sulfate and glucuronide phytoestrogen conjugates in human urine using isotope dilution liquid chromatography-tandem mass spectrometry with [13C3] isoflavone internal standards
Clarke DB, et al.
Analytical Biochemistry, 309(1), 158-172 (2002)
Denis M Callewaert et al.
Methods in molecular biology (Clifton, N.J.), 610, 435-449 (2009-12-17)
Isoprostanes are a group of prostaglandin-like compounds that are derived in vivo primarily by free radical-mediated peroxidation of arachidonic acid within phospholipids. The resulting isoprostane moieties are rapidly hydrolyzed, metabolized, and excreted. It is now well recognized that isoprostane levels
GC-MS methods for metabolic profiling of microbial fermentation products of dietary polyphenols in human and in vitro intervention studies
Grun CH, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 871(2), 212-219 (2008)
Tissue distribution of quercetin in rats and pigs
de Boer VCJ, et al.
The Journal of Nutrition, 135(7), 1718-1725 (2005)
Maaike M Appeldoorn et al.
The Journal of nutrition, 139(8), 1469-1473 (2009-06-06)
Intervention studies with procyanidin (PC)-rich extracts and products such as cocoa and wine suggest protective effects of PC against cardiovascular diseases. However, there is no consensus on the absorption and metabolism of PC dimers. Interestingly, nothing is known about the
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service