A7089
Accumax™ solution
sterile-filtered, suitable for cell culture
Synonym(s):
Cell detachment solution
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About This Item
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sterility
sterile-filtered
Quality Level
form
solution
concentration
1 ×
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
pH
6.8-7.8
shipped in
dry ice
storage temp.
−20°C
Application
TM=["Accumax"] solution has been used:
- for detaching single walled carbon nanotubes from the extracellular matrix (ECM) in epithelial cells
- for dissociation of retinal pigmented epithelial cells prior to passaging
- for dissociation of islets from pancreas
Accumax has proven effective in dissociating clumped cells in suspension cultures of hybridomas, CHO, BHK, 293, COS and Sf9 cells. The solution performed extremely well in dissociating cell counting, viral transfection assays, cell sorting, flow cytometry, and bioreactor scale-up. It has also been used to create single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue, and the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.
Biochem/physiol Actions
Accumax is a cell detachment solution consisting of proteolytic and collagenolytic enzymes. Each bottle has 100 mL of 1x Accumax enzymes in Dulbecco′s PBS. There are no mammalian or bacterial-derived products found in Accumax.
Features and Benefits
Ready-to-use sterile liquid for in vitro cell applications
Caution
Accumax is stable when stored at -20°C. After thawing, the product can be stored for up to 2 months at 4°C.
Formula variant
Prepared in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4). Refer to Product Data Sheet on the website for product usage information.
Preparation Note
This product is supplied as a sterile, ready-to-use frozen liquid. Accumax should be thawed at 37°C before use, and added at equal volume to a sample of clumped cells. Allow 5-10 minutes at 37°C for incubation and count cells as you would normally.
Legal Information
Accumax is a trademark of Innovative Cell Technologies, Inc.
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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