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M16 Medium

Name: M16 Medium
Brand: SIGMA

With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered

Synonym(s):

Cell Culture Medium, Cell Culture Medium M16, M16 Medium Solution

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

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Properties

Quality Segment

300

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | embryo: suitable

impurities

endotoxin, tested

components

NaHCO₃: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

Description

Application

M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

Preparation Note

Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.

Analysis Note

Presence of particulates in solution will not affect product′s performance.

This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.

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This Item	M7167	CB_88052038	WH0010626M1
Sigma-Aldrich M7292 M16 Medium	Sigma-Aldrich M7167 M2 medium	BM16 human cell line	Sigma-Aldrich WH0010626M1 Monoclonal Anti-TRIM16 antibody produced in mouse
description With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered	description With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture	description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., IHW Number: 9038, 88052038	description clone 5F4, purified immunoglobulin, buffered aqueous solution
technique(s) cell culture \| embryo: suitable	technique(s) cell culture \| embryo: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) western blot: 1-5 μg/mL, immunoprecipitation (IP): suitable, indirect ELISA: suitable
sterility sterile-filtered	sterility sterile-filtered	sterility -	sterility -
form liquid	form liquid	form -	form buffered aqueous solution
components NaHCO₃: 2.101 g/L phenol red: 0.0106 g/L sodium pyruvate: 0.0363 g/L glucose: 1.0 g/L (Dextro)	components HEPES: 5.42726 g/L NaHCO₃: 0.35 g/L glucose: 1.0 g/L (Dextro) phenol red: 0.0106 g/L sodium pyruvate: 0.0363 g/L	components -	components -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. -140 to -196°C	storage temp. −20°C
shipped in ambient	shipped in ambient	shipped in dry ice	shipped in dry ice

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Storage Class

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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