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M2 medium

Name: M2 medium
Brand: SIGMA

With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture

Synonym(s):

Cell Culture M2, Cell Culture Medium, M2 Medium Solution

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00283761

Pricing and availability is not currently available.

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Properties

Quality Segment

300

sterility

sterile-filtered

form

liquid

storage condition

protect from light

technique(s)

cell culture | embryo: suitable

impurities

endotoxin, tested

components

HEPES: 5.42726 g/L
NaHCO₃: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L

shipped in

ambient

storage temp.

2-8°C

Description

Application

Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.

Preparation Note

Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.

Analysis Note

Presence of particulates in solution will not affect product′s performance.

This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.

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This Item	M7292	A3311	HPA063728
Sigma-Aldrich M7167 M2 medium	Sigma-Aldrich M7292 M16 Medium	Sigma-Aldrich A3311 Bovine Serum Albumin	Sigma-Aldrich HPA063728 Anti-PROM2 antibody produced in rabbit
description With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture	description With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered	description powder, BioXtra	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
sterility sterile-filtered	sterility sterile-filtered	sterility -	sterility -
form liquid	form liquid	form powder	form buffered aqueous glycerol solution
technique(s) cell culture \| embryo: suitable	technique(s) cell culture \| embryo: suitable	technique(s) cell culture \| embryo: suitable	technique(s) immunohistochemistry: 1:500-1:1000, immunofluorescence: 0.25-2 μg/mL
components HEPES: 5.42726 g/L NaHCO₃: 0.35 g/L glucose: 1.0 g/L (Dextro) phenol red: 0.0106 g/L sodium pyruvate: 0.0363 g/L	components NaHCO₃: 2.101 g/L phenol red: 0.0106 g/L sodium pyruvate: 0.0363 g/L glucose: 1.0 g/L (Dextro)	components -	components -
shipped in ambient	shipped in ambient	shipped in ambient	shipped in wet ice
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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